Enhanced nicotine-seeking behavior following pre-exposure to repeated cocaine is accompanied by changes in BDNF in the nucleus accumbens of rats

Autor(es)

Leao, Rodrigo M. ^{[1, 2]} ; Cruz, Fabio C. ^[1] ; Carneiro-de-Oliveira, Paulo E. ^{[1, 2]} ; Rossetto, Daniella B. ^[3] ; Valentini, Sandro R. ^[3] ; Zanelli, Cleslei F. ^[3] ; Planeta, Cleopatra S. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Pharmacol Lab, Sch Pharmaceut Sci, BR-14801902 Sao Paulo - Brazil

^[2] Joint Grad Program Physiol Sci UFSCar UNESP, Sao Paulo - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Biol Sci, Sch Pharmaceut Sci, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Pharmacology Biochemistry and Behavior; v. 104, p. 169-176, MAR 2013.
• Citações Web of Science: 7

Resumo

We investigated the behavioral and molecular interactions between cocaine and nicotine, through evaluating locomotor activity, nicotine intravenous self-administration and gene expression. Locomotor sensitization was induced in male Wistar rats by repeated cocaine (20 mg/kg; i.p.) or saline injections once a day over 7 days. Three days after the last injection, rats were challenged with either saline or cocaine (15 mg/kg; i.p.) and the locomotor activity was measured. The very next day animals received either saline or nicotine (0.4 mg/kg; s.c.) and the locomotor cross-sensitization was tested. Animals were then prepared with intrajugular catheters for nicotine self-administration. Nicotine self-administration patterns were evaluated using fixed or progressive ratio schedules of reinforcement and a 24-h unlimited access binge. Immediately after the binge sessions animals were decapitated, the brains were removed and the nucleus accumbens was dissected. The dynorphin (DYN), mu-opioid receptor (mu opioid), neuropeptide Y (NPY), brain-derived neurotrophic factor (BDNF), tropomyosin-related tyrosine kinase B receptor (TrkB) and corticotropin-releasing factor receptor type 1 (CRF-R1) gene expression were measured by the reverse transcription-polymerase chain reaction (RT-PCR). Pretreatment with cocaine caused sensitization of cocaine motor response and locomotor cross-sensitization with nicotine. In the self-administration experiments repeated cocaine administration caused an increase in the nicotine break point and nicotine intake during a 24 h binge session. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/07469-9 - Interação entre etanol e nicotina ao longo da ontogênese: avaliação de parâmetros da autoadministração e expressão gênica no sistema mesocorticolímbico
• Beneficiário: Cleopatra da Silva Planeta
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/57541-2 - Efeitos da cafeina na transmissao serotonergica e dopaminergica em ratos adultos e adolescentes.
• Beneficiário: Ana Paula Marques Possi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/10691-2 - Estresse crônico e auto-administração de nicotina: envolvimento da via de transdução PKA, CREB e ERK no núcleo acumbens e córtex pré-frontal
• Beneficiário: Rodrigo Molini Leão
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto