In Vivo Genotoxicity and Oxidative Stress Evaluation of an Ethanolic Extract from Piquia (Caryocar villosum) Pulp

Autor(es)

Almeida, Mara Ribeiro ^[1] ; Aissa, Alexandre Ferro ^[2] ; Ursula Hermogenes Gomes, Tarsila Daysy ^[1] ; Castania Darin, Joana D'Arc ^[1] ; Chiste, Renan Campos ^[3] ; Mercadante, Adriana Zerlotti ^[3] ; Greggi Antunes, Lusania Maria ^[1] ; Pires Bianchi, Maria Lourdes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MEDICINAL FOOD; v. 16, n. 3, p. 268-271, MAR 2013.
• Citações Web of Science: 5

Resumo

In this study, the ethanolic extract obtained from piquia pulp was assessed for genotoxicity and oxidative stress by employing the micronucleus test in bone marrow and peripheral blood cells in addition to comet, thiobarbituric-acid-reactive substances (TBARS), and reduced glutathione assays in the liver, kidney, and heart. Additionally, phytochemical analyses were performed to identify and quantify the chemical constituents of the piquia extract. Wistar rats were treated by gavage with an ethanolic extract from piquia pulp (75 mg/kg body weight) for 14 days, and 24 h prior to euthanasia, they received an injection of saline or doxorubicin (15 mg/kg body weight, intraperoneally). The results demonstrated that piquia extract at the tested dose was genotoxic but not mutagenic, and it increased the TBARS levels in the heart. Further studies are required to fully elucidate how the properties of ethanolic extract of piquia pulp can affect human health. (AU)

• Processo FAPESP: 09/15692-0 - Avaliação da citotoxicidade, genotoxicidade, antigenotoxicidade e expressão dos genes TP53 e Ephx2 em ratos tratados com Caryocar villosum
• Beneficiário: Mara Ribeiro de Almeida
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 05/59552-6 - Avaliação integrada da estabilidade e propriedades funcionais de pigmentos naturais de alimentos
• Beneficiário: Adriana Zerlotti Mercadante
• Linha de fomento: Auxílio à Pesquisa - Temático