Evaluation of the interactions of DNA with the textile dyes Disperse Orange 1 and Disperse Red 1 and their electrolysis products using an electrochemical biosensor

Autor(es)

Uliana, Carolina Venturini ^[1] ; Garbellini, Gustavo Stoppa ^[1] ; Yamanaka, Hideko ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Chem Araraquara, Dept Analyt Chem, BR-14800900 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SENSORS AND ACTUATORS B-CHEMICAL; v. 178, p. 627-635, MAR 1 2013.
• Citações Web of Science: 19

Resumo

A disposable pencil graphite electrode modified with dsDNA was used in combination with square wave voltammetry in order to evaluate the interaction of DNA with the textile dyes Disperse Orange 1 (DO1) and Disperse Red 1 (DR1), and with the products of their electrolysis. Significant changes in the characteristic oxidation peaks of the guanine and adenine moieties of immobilized dsDNA were observed after incubation of the modified electrode for 180 s in solutions of the dyes in their original forms. The same was observed using the electrolysis products obtained by oxidation and reduction conversions. The oxidation peak currents of the guanine and adenine moieties decreased when the concentrations of DO1 and DR1 were increased up to 5.0 x 10(-6) and 1.0 x 10(-6) mol L-1, respectively; the signal decreases were more pronounced after interaction with the oxidized dyes, compared to the reduced compounds. The interactions between DNA and DO1, DR1, and the electrolyzed dyes were further investigated by UV-vis spectrophotometry in solution, and different effects such as hypochromism and hyperchromism were observed in the resulting DNA spectra. The investigated interactions showed clear evidence of changes in the DNA structure, and suggested a predominant intercalation mode leading to damage in the biomolecule. (c) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/08161-8 - Avaliação de interações entre pesticidas organofosforados e DNA empregando biossensor eletroquímico
• Beneficiário: Gustavo Stoppa Garbellini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/08990-1 - Estudos da lesão ao DNA por corantes têxteis e da capacidade protetora de flavonóides empregando biossensor eletroquímico
• Beneficiário: Carolina Venturini Uliana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/10449-7 - Avaliação da ocorrência, toxicidade/genotoxicidade e processos para degradação de corantes em efluentes e águas superficiais
• Beneficiário: Maria Valnice Boldrin
• Modalidade de apoio: Auxílio à Pesquisa - Temático