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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the interactions of DNA with the textile dyes Disperse Orange 1 and Disperse Red 1 and their electrolysis products using an electrochemical biosensor

Full text
Author(s):
Uliana, Carolina Venturini [1] ; Garbellini, Gustavo Stoppa [1] ; Yamanaka, Hideko [1]
Total Authors: 3
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Chem Araraquara, Dept Analyt Chem, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 178, p. 627-635, MAR 1 2013.
Web of Science Citations: 19
Abstract

A disposable pencil graphite electrode modified with dsDNA was used in combination with square wave voltammetry in order to evaluate the interaction of DNA with the textile dyes Disperse Orange 1 (DO1) and Disperse Red 1 (DR1), and with the products of their electrolysis. Significant changes in the characteristic oxidation peaks of the guanine and adenine moieties of immobilized dsDNA were observed after incubation of the modified electrode for 180 s in solutions of the dyes in their original forms. The same was observed using the electrolysis products obtained by oxidation and reduction conversions. The oxidation peak currents of the guanine and adenine moieties decreased when the concentrations of DO1 and DR1 were increased up to 5.0 x 10(-6) and 1.0 x 10(-6) mol L-1, respectively; the signal decreases were more pronounced after interaction with the oxidized dyes, compared to the reduced compounds. The interactions between DNA and DO1, DR1, and the electrolyzed dyes were further investigated by UV-vis spectrophotometry in solution, and different effects such as hypochromism and hyperchromism were observed in the resulting DNA spectra. The investigated interactions showed clear evidence of changes in the DNA structure, and suggested a predominant intercalation mode leading to damage in the biomolecule. (c) 2013 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/08161-8 - Evaluation of organophosphate pesticides and DNA interactions using electrochemical biosensor
Grantee:Gustavo Stoppa Garbellini
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 08/08990-1 - Studies of DNA damage by textile dyes and the protective capacity of flavonoids using electrochemical biosensor
Grantee:Carolina Venturini Uliana
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 08/10449-7 - Assessment of occurrence, toxicity/genotoxicity and degradation processes of dyes in effluents and surface water
Grantee:Maria Valnice Boldrin
Support Opportunities: Research Projects - Thematic Grants