Phenolic compounds from Rosemary (Rosmarinus officinalis L.) attenuate oxidative stress and reduce blood cholesterol concentrations in diet-induced hypercholesterolemic rats

Autor(es)

Afonso, Milessa S. ^{[1, 2]} ; Silva, Ana Mara de O. ^[1] ; Carvalho, Eliane B. T. ^[1] ; Rivelli, Diogo P. ^[3] ; Barros, Silvia B. M. ^[3] ; Rogero, Marcelo M. ^[4] ; Lottenberg, Ana Maria ^[2] ; Torres, Rosangela P. ^[1] ; Mancini-Filho, Jorge ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Sci, Lipids Lab LIM 10, BR-01246000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRITION & METABOLISM; v. 10, FEB 2 2013.
• Citações Web of Science: 44

Resumo

Background: Phenolic compounds combine antioxidant and hypocholesterolemic activities and, consequently, are expected to prevent or minimize cardiometabolic risk. Methods: To evaluate the effect of an aqueous extract (AQ) and non-esterified phenolic fraction (NEPF) from rosemary on oxidative stress in diet-induced hypercholesterolemia, 48 male 4-week old Wistar rats were divided into 6 groups: 1 chow diet group (C) and 5 hypercholesterolemic diet groups, with 1 receiving water (HC), 2 receiving AQ at concentrations of 7 and 140 mg/kg body weight (AQ70 and AQ140, respectively), and 2 receiving NEPF at concentrations of 7 and 14 mg/kg body weight (NEPF7 and NEPF14, respectively) by gavage for 4 weeks. Results: In vitro, both AQ and NEPF had remarkable antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) assay, which was similar to BHT. In vivo, the group that received AQ at 70 mg/kg body weight had lower serum total cholesterol (-39.8%), non-HDL-c (-44.4%) and thiobarbituric acid reactive substance (TBARS) levels (-37.7%) compared with the HC group. NEPF (7 and 14 mg/kg) reduced the tissue TBARS levels and increased the activity of tissular antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). Neither AQ nor NEPF was able to ameliorate the alterations in the hypercholesterolemic diet-induced fatty acid composition in the liver. Conclusions: These data suggest that phenolic compounds from rosemary ameliorate the antioxidant defense in different tissues and attenuate oxidative stress in diet-induced hypercholesterolemic rats, whereas the serum lipid profile was improved only in rats that received the aqueous extract. (AU)

• Processo FAPESP: 08/51333-1 - Avaliacao do efeito do extrato aquoso e da fracao fenolica livre do alecrim (rosmarinus officinalis l.) sobre o estado antioxidante e o perfil lipidico em ratos com hipercolesterolemia induzida pela dieta.
• Beneficiário: Milessa da Silva Afonso
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/54319-0 - Avaliacao do efeito do extrato aquoso e da fracao fenolica do alecrim (rosmarinus officinalis l.) sobre o estado antioxidante e o perfil lipidico em ratos hipercolesterolemicos
• Beneficiário: Jorge Mancini Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular