Suppressive effect of low-level laser therapy on tracheal hyperresponsiveness and lung inflammation in rat subjected to intestinal ischemia and reperfusion

Autor(es)

de Lima, Flavia Mafra ^[1] ; Vitoretti, Luana ^[2] ; Coelho, Fernando ^[3] ; Albertini, Regiane ^[1] ; Breithaupt-Faloppa, Ana Cristina ^[4] ; de Lima, Wothan Tavares ^[2] ; Aimbire, Flavio ^[5]

Afiliação do(s) autor(es)

^[1] Univ Nove Julho, Dept Rehabil Sci, UNINOVE, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Farmacol, Lab Fisiopatol Expt, BR-05508900 Sao Paulo - Brazil

^[3] Inst Quim, Dept Bioquim, Lab Radicais Livres, BR-0550800 Sao Paulo - Brazil

^[4] Hosp Clin FMUSP, Lab Cirurgia Cardiovasc & Fisiopatol Circulacao L, BR-01246093 Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Sci & Technol, UNIFESP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 28, n. 2, p. 551-564, FEB 2013.
• Citações Web of Science: 10

Resumo

Intestinal ischemia and reperfusion (i-I/R) is an insult associated with acute respiratory distress syndrome (ARDS). It is not known if pro- and anti-inflammatory mediators in ARDS induced by i-I/R can be controlled by low-level laser therapy (LLLT). This study was designed to evaluate the effect of LLLT on tracheal cholinergic reactivity dysfunction and the release of inflammatory mediators from the lung after i-I/R. Anesthetized rats were subjected to superior mesenteric artery occlusion (45 min) and killed after clamp release and preestablished periods of intestinal reperfusion (30 min, 2 or 4 h). The LLLT (660 nm, 7.5 J/cm(2)) was carried out by irradiating the rats on the skin over the right upper bronchus for 15 and 30 min after initiating reperfusion and then euthanizing them 30 min, 2, or 4 h later. Lung edema was measured by the Evans blue extravasation technique, and pulmonary neutrophils were determined by myeloperoxidase (MPO) activity. Pulmonary tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10), intercellular adhesion molecule-1 (ICAM-1), and isoform of NO synthase (iNOS) mRNA expression were analyzed by real-time PCR. TNF-alpha, IL-10, and iNOS proteins in the lung were measured by the enzyme-linked immunoassay technique. LLLT (660 nm, 7.5 J/cm(2)) restored the tracheal hyperresponsiveness and hyporesponsiveness in all the periods after intestinal reperfusion. Although LLLT reduced edema and MPO activity, it did not do so in all the postreperfusion periods. It was also observed with the ICAM-1 expression. In addition to reducing both TNF-alpha and iNOS, LLLT increased IL-10 in the lungs of animals subjected to i-I/R. The results indicate that LLLT can control the lung's inflammatory response and the airway reactivity dysfunction by simultaneously reducing both TNF-alpha and iNOS. (AU)

• Processo FAPESP: 08/08838-5 - Estudo do mecanismo de ação antiinflamatória do laser de baixa potência em modelo experimental de inflamação pulmonar alérgica e sistêmica
• Beneficiário: Flávio Aimbire Soares de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/08048-4 - Estudo da ação antiinflamatória do laser de baixa potência sobre a reatividade brônquica e a inflamação pulmonar induzida por isquemia e reperfusão intestinal
• Beneficiário: Flávia Mafra de Lima
• Modalidade de apoio: Bolsas no Brasil - Mestrado