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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Suppressive effect of low-level laser therapy on tracheal hyperresponsiveness and lung inflammation in rat subjected to intestinal ischemia and reperfusion

Full text
Author(s):
de Lima, Flavia Mafra [1] ; Vitoretti, Luana [2] ; Coelho, Fernando [3] ; Albertini, Regiane [1] ; Breithaupt-Faloppa, Ana Cristina [4] ; de Lima, Wothan Tavares [2] ; Aimbire, Flavio [5]
Total Authors: 7
Affiliation:
[1] Univ Nove Julho, Dept Rehabil Sci, UNINOVE, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Farmacol, Lab Fisiopatol Expt, BR-05508900 Sao Paulo - Brazil
[3] Inst Quim, Dept Bioquim, Lab Radicais Livres, BR-0550800 Sao Paulo - Brazil
[4] Hosp Clin FMUSP, Lab Cirurgia Cardiovasc & Fisiopatol Circulacao L, BR-01246093 Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Sci & Technol, UNIFESP, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Lasers in Medical Science; v. 28, n. 2, p. 551-564, FEB 2013.
Web of Science Citations: 10
Abstract

Intestinal ischemia and reperfusion (i-I/R) is an insult associated with acute respiratory distress syndrome (ARDS). It is not known if pro- and anti-inflammatory mediators in ARDS induced by i-I/R can be controlled by low-level laser therapy (LLLT). This study was designed to evaluate the effect of LLLT on tracheal cholinergic reactivity dysfunction and the release of inflammatory mediators from the lung after i-I/R. Anesthetized rats were subjected to superior mesenteric artery occlusion (45 min) and killed after clamp release and preestablished periods of intestinal reperfusion (30 min, 2 or 4 h). The LLLT (660 nm, 7.5 J/cm(2)) was carried out by irradiating the rats on the skin over the right upper bronchus for 15 and 30 min after initiating reperfusion and then euthanizing them 30 min, 2, or 4 h later. Lung edema was measured by the Evans blue extravasation technique, and pulmonary neutrophils were determined by myeloperoxidase (MPO) activity. Pulmonary tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10), intercellular adhesion molecule-1 (ICAM-1), and isoform of NO synthase (iNOS) mRNA expression were analyzed by real-time PCR. TNF-alpha, IL-10, and iNOS proteins in the lung were measured by the enzyme-linked immunoassay technique. LLLT (660 nm, 7.5 J/cm(2)) restored the tracheal hyperresponsiveness and hyporesponsiveness in all the periods after intestinal reperfusion. Although LLLT reduced edema and MPO activity, it did not do so in all the postreperfusion periods. It was also observed with the ICAM-1 expression. In addition to reducing both TNF-alpha and iNOS, LLLT increased IL-10 in the lungs of animals subjected to i-I/R. The results indicate that LLLT can control the lung's inflammatory response and the airway reactivity dysfunction by simultaneously reducing both TNF-alpha and iNOS. (AU)

FAPESP's process: 08/08838-5 - Study of anti-inflammatory action mechanism of Low Level Laser on experimental model of allergic and systemic pulmonary inflammation.
Grantee:Flávio Aimbire Soares de Carvalho
Support Opportunities: Regular Research Grants
FAPESP's process: 08/08048-4 - Study of anti-inflammatory action of low level laser therapy on bronchial reactivity and lung inflammation induced by intestinal reperfusion ischemia in rats
Grantee:Flávia Mafra de Lima
Support Opportunities: Scholarships in Brazil - Master