Involvement of N-methyl-D-aspartate glutamate receptor and nitric oxide in cardiovascular responses to dynamic exercise in rats

Autor(es)

Camargo, Laura H. A. ^[1] ; Alves, Fernando H. F. ^[2] ; Biojone, Caroline ^[2] ; Correa, Fernando M. A. ^[2] ; Resstel, Leonardo B. M. ^[2] ; Crestani, Carlos C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Pharmacol Lab, Dept Nat Active Principles & Toxico, BR-14801902 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Pharmacology; v. 713, n. 1-3, p. 16-24, AUG 5 2013.
• Citações Web of Science: 9

Resumo

Dynamic exercise evokes sustained cardiovascular responses, which are characterized by arterial pressure and heart rate increases. Although it is well accepted that there is central nervous system mediation of cardiovascular adjustments during exercise, information on the role of neural pathways and signaling mechanisms is limited. It has been reported that glutamate, by acting on NMDA receptors, evokes the release of nitric oxide through activation of neuronal nitric oxide synthase (nNOS) in the brain. In the present study, we tested the hypothesis that NMDA receptors and nNOS are involved in cardiovascular responses evoked by an acute bout of exercise on a rodent treadmill. Moreover, we investigated possible central sites mediating control of responses to exercise through the NMDA receptor-nitric oxide pathway. Intraperitoneal administration of the selective NMDA glutamate receptor antagonist dizocilpine maleate (MK-801) reduced both the arterial pressure and heart rate increase evoked by dynamic exercise. Intraperitoneal treatment with the preferential nNOS inhibitor 7-nitroindazole reduced exercise-evoked tachycardiac response without affecting the pressor response. Moreover, treadmill running increased NO formation in the medial prefrontal cortex (MPFC), bed nucleus of the stria teminalis (BNST) and periaqueductal gray (PAG), and this effect was inhibited by systemic pretreatment with MK-801. Our findings demonstrate that NMDA receptors and nNOS mediate the tachycardiac response to dynamic exercise, possibly through an NMDA receptor-NO signaling mechanism. However, NMDA receptors, but not nNOS, mediate the exercise-evoked pressor response. The present results also provide evidence that MPFC, BNST and PAG may modulate physiological adjustments during dynamic exercise through NMDA receptor-NO signaling. (c) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/13299-9 - O sistema noradrenérgico do núcleo leito da estria terminal modulando a resposta emocional condicionada contextual: possível interação com o CRF e a neurotransmissão glutamatérgica
• Beneficiário: Sara Cristina Hott
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/16192-8 - Efeitos cardiovasculares decorrentes da administração crônica de testosterona e cocaína em ratos
• Beneficiário: Carlos Cesar Crestani
• Modalidade de apoio: Auxílio à Pesquisa - Regular