Nucleotide and phylogenetic analysis of human papillomavirus types 6 and 11 isolated from recurrent respiratory papillomatosis in Brazil

Autor(es):Mostrar menos -

Adum de Matos, Renata Prandini ^[1] ; Sichero, Laura ^[2] ; Mansur, Isabela Mazuco ^[1] ; do Bonfim, Caroline Measso ^[1] ; Bittar, Cintia ^[1] ; Nogueira, Rodrigo Lacerda ^[3] ; Kuepper, Daniel Salgado ^[3] ; Pereira Valera, Fabiana Cardoso ^[3] ; Nogueira, Mauricio Lacerda ^[4] ; Villa, Luisa Lina ^{[5, 2, 6]} ; Calmon, Marilia Freitas ^[1] ; Rahal, Paula ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Dept Biol, Inst Biosci Language & Literature & Exact Sci, IBILCE, BR-15054010 Sao Paulo - Brazil

^[2] Canc Inst State Sao Paulo, Ctr Translat Res Oncol, ICESP, BR-01246000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Ophthalmol Otorhinolaryngol & Surg Head Neck, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] FAMERP, Sch Med Sao Jose do Rio Preto, Lab Res Virol, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, BR-05403900 Sao Paulo - Brazil

^[6] Santa Casa Coll Med Sci, HPV Inst, BR-01223001 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 16, p. 282-289, JUN 2013.
• Citações Web of Science: 12

Resumo

There are few studies about the distribution of natural molecular variants of low-risk HPVs. Our aim was to evaluate the E6 early gene variability among HPV-6 and HPV-11 isolates detected in recurrent respiratory papillomatosis (RRP) samples obtained in a cohort of Brazilian patients. We also performed a phylogenetic analysis in order to compare nucleotide sequences identified in our study with previously reported isolates from different anatomic sites (laryngeal papillomas, genital warts, cervical cancer and anal swabs) obtained from other parts of the world to determine the phylogenetic relationships of variants detected in Brazil. The complete coding region of the E6 gene of 25 samples was cloned and sequenced: 18 isolates of HPV-6 (72%) and 7 isolates of HPV-11 (28%). A total of four different HPV-6 genomic variants and two HPV-11 genomic variants was identified. It was not possible to correlate specific variants with disease severity. Phylogenetic trees for both HPV types were constructed enclosing both E6 sequences detected in our study and formerly published sequences. In both phylogenetic trees, the sequences from Brazil did not group together. We could not establish a geographical association between HPV-6 or HPV-11 variants, unlike HPV-16 and HPV-18. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/57889-1 - Instituto de Ciência e Tecnologia para o Estudo das Doenças Associadas ao Papilomavírus
• Beneficiário: Luisa Lina Villa
• Modalidade de apoio: Auxílio à Pesquisa - Temático