Assessment of the chemopreventive effect of casearin B, a clerodane diterpene extracted from Casearia sylvestris (Salicaceae)

Autor(es)

Prieto, Aline M. ^[1] ; dos Santos, Andre G. ^[2] ; Oliveira, Ana Paula S. ^[3] ; Cavalheiro, Alberto J. ^[4] ; Silva, Dulce H. S. ^[4] ; Bolzani, Vanderlan S. ^[4] ; Varanda, Eliana Ap. ^[3] ; Soares, Christiane P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Nat Principles & Toxicol, Araraquara, SP - Brazil

^[3] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Inst Chem, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Chemical Toxicology; v. 53, p. 153-159, MAR 2013.
• Citações Web of Science: 14

Resumo

Studies have shown that Casearia sylvestris compounds protect DNA from damage both in vitro and in vivo. Complementarily, the aim of the present study was to assess the chemopreventive effect of casearin B (CASB) against DNA damage using the Ames test, the comet assay and the DCFDA antioxidant assay. The genotoxicity was assessed by the comet assay in HepG2 cells. CASS was genotoxic at concentrations higher than 030 mu M when incubated with the FPG (formamidopyrimidine-DNA glycosylase) enzyme. For the antigenotoxicity comet assay, CASB protected the DNA from damage caused by H2O2 in the HepG2 cell line in concentrations above 0.04 mu M with post-treatment, and above 0.08 mu M with pre-treatment. CASB was not mutagenic (Ames test) in TA 98 and TA 102. In the antimutagenicity assays, the compound was a strong inhibitor against aflatoxin B1 (AFB) in TA 98 (>88.8%), whereas it was moderate (42.7-59.4%) inhibitor against mytomicin C (MMC) in TA 102. Additionally, in the antioxidant assay using DCFDA, CASB reduced reactive oxygen species (ROS) generated by H2O2. In conclusion, CASB was genotoxic to HepG2 cells at high concentrations; was protective of DNA at low concentrations, as shown by the Ames test and comet assay; and was also antioxidant. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 03/02176-7 - Conservação e uso sustentável da diversidade do Cerrado e da Mata Atlântica: diversidade química e prospecção de medicamentos potenciais - fase II
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 09/52481-7 - Estudo da acao reparadora de dna produzida pelo extrato etanolico e substancias isoladas de casearia sylvestris
• Beneficiário: Christiane Pienna Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/07932-7 - Busca de compostos naturais antitumorais, antioxidantes, antiinflamatórios, antidiabéticos, inibidores de acetilcolinesterase e mieloperoxidase no Cerrado e na Mata Atlântica
• Beneficiário: Dulce Helena Siqueira Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 08/58908-0 - Avaliação de reparo de DNA e atividade antioxidante do extrato etanólico e substâncias isoladas de Casearia sylvestris
• Beneficiário: Aline Miranda de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto