| Full text | |
| Author(s): |
Prieto, Aline M.
[1]
;
dos Santos, Andre G.
[2]
;
Oliveira, Ana Paula S.
[3]
;
Cavalheiro, Alberto J.
[4]
;
Silva, Dulce H. S.
[4]
;
Bolzani, Vanderlan S.
[4]
;
Varanda, Eliana Ap.
[3]
;
Soares, Christiane P.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Nat Principles & Toxicol, Araraquara, SP - Brazil
[3] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, SP - Brazil
[4] Univ Estadual Paulista, UNESP, Inst Chem, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, Araraquara, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Food and Chemical Toxicology; v. 53, p. 153-159, MAR 2013. |
| Web of Science Citations: | 14 |
| Abstract | |
Studies have shown that Casearia sylvestris compounds protect DNA from damage both in vitro and in vivo. Complementarily, the aim of the present study was to assess the chemopreventive effect of casearin B (CASB) against DNA damage using the Ames test, the comet assay and the DCFDA antioxidant assay. The genotoxicity was assessed by the comet assay in HepG2 cells. CASS was genotoxic at concentrations higher than 030 mu M when incubated with the FPG (formamidopyrimidine-DNA glycosylase) enzyme. For the antigenotoxicity comet assay, CASB protected the DNA from damage caused by H2O2 in the HepG2 cell line in concentrations above 0.04 mu M with post-treatment, and above 0.08 mu M with pre-treatment. CASB was not mutagenic (Ames test) in TA 98 and TA 102. In the antimutagenicity assays, the compound was a strong inhibitor against aflatoxin B1 (AFB) in TA 98 (>88.8%), whereas it was moderate (42.7-59.4%) inhibitor against mytomicin C (MMC) in TA 102. Additionally, in the antioxidant assay using DCFDA, CASB reduced reactive oxygen species (ROS) generated by H2O2. In conclusion, CASB was genotoxic to HepG2 cells at high concentrations; was protective of DNA at low concentrations, as shown by the Ames test and comet assay; and was also antioxidant. (C) 2012 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 03/02176-7 - Conservation and sustainable use of the diversity from Cerrado and Atlantic Forest: chemical diversity and prospecting for potential drugs - phase II |
| Grantee: | Vanderlan da Silva Bolzani |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 04/07932-7 - Search for potential antitumoral, antioxidant, antiinflammatory, antidiabetic, acetylcholinesterase and mieloperoxidase inhibitory natural compounds from Cerrado and Atlantic Forest |
| Grantee: | Dulce Helena Siqueira Silva |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |