Isolation and Characterization of Phytotoxic Compounds Produced by Streptomyces sp AMC 23 from Red Mangrove (Rhizophora mangle)

Autor(es)

Crevelin, Eduardo Jose ^[1] ; Canova, Sarah Pigato ^[2] ; Melo, Itamar Soares ^[2] ; Zucchi, Tiago Domingues ^[2] ; da Silva, Rafael Eduardo ^[2] ; Beraldo Moraes, Luiz Alberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Mass Spectrometry Appl Nat Prod Chem, Dept Chem, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Brazilian Agr Res Corp EMBRAPA, Lab Environm Microbiol, Jaguariuna, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 171, n. 7, p. 1602-1616, DEC 2013.
• Citações Web of Science: 8

Resumo

Natural products produced by microorganisms have been utilized as sources of new drugs possessing a wide range of agrochemical and pharmacological activities. During our research on Actinomycetes from Brazilian mangroves, the ethyl acetate extract of Streptomyces sp. AMC 23 isolated from the red mangrove (Rhizophora mangle) rhizosphere produced a highly active compound against the microalga Chlorella vulgaris, often used to assess the phytotoxic activity. As a result, the bioassay-guided fractionation led to the isolation of the mixture of the known compounds bafilomycin B-1 and bafilomycin B-2. The chemical structures of bafilomycin B-1 and bafilomycin B-2 were established based on their spectroscopic data by infrared (IR), mass spectrometry (MS), H-1 nuclear magnetic resonance (NMR), gradient-enhanced heteronuclear multiple quantum coherence (gHMQC), and gradient-enhanced heteronuclear multiple-bond connectivity (gHMBC) as well as comparison with reference data from the literature. Moreover, it was also possible to identify other bafilomycins using non-chromatographic-dependent techniques (Tandem mass spectrometry). Additionally, this is the first report on the phytotoxic activity of bafilomycin B-1. (AU)

• Processo FAPESP: 11/14333-6 - Diversidade e taxonomia polifásica de actinobactérias isoladas de áreas de reflorestamento
• Beneficiário: Tiago Domingues Zucchi
• Modalidade de apoio: Bolsas no Brasil - Programa BIOTA - Jovens Pesquisadores
• Processo FAPESP: 11/50243-1 - Diversidade e taxonomia polifásica de actinobactérias isoladas de áreas de reflorestamento
• Beneficiário: Tiago Domingues Zucchi
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores
• Processo FAPESP: 12/01904-8 - Diversidade de micromonosporaceae em solos de reflorestamento do estado de são paulo
• Beneficiário: Rafael Eduardo da Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/53000-0 - Isolamento e caracterizacao de micoherbicidas com atividade inibidora da fotossintese empregando a espectrometria de massas.
• Beneficiário: Eduardo José Crevelin
• Modalidade de apoio: Bolsas no Brasil - Doutorado