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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and Characterization of Phytotoxic Compounds Produced by Streptomyces sp AMC 23 from Red Mangrove (Rhizophora mangle)

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Author(s):
Crevelin, Eduardo Jose [1] ; Canova, Sarah Pigato [2] ; Melo, Itamar Soares [2] ; Zucchi, Tiago Domingues [2] ; da Silva, Rafael Eduardo [2] ; Beraldo Moraes, Luiz Alberto [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Lab Mass Spectrometry Appl Nat Prod Chem, Dept Chem, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Brazilian Agr Res Corp EMBRAPA, Lab Environm Microbiol, Jaguariuna, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Applied Biochemistry and Biotechnology; v. 171, n. 7, p. 1602-1616, DEC 2013.
Web of Science Citations: 8
Abstract

Natural products produced by microorganisms have been utilized as sources of new drugs possessing a wide range of agrochemical and pharmacological activities. During our research on Actinomycetes from Brazilian mangroves, the ethyl acetate extract of Streptomyces sp. AMC 23 isolated from the red mangrove (Rhizophora mangle) rhizosphere produced a highly active compound against the microalga Chlorella vulgaris, often used to assess the phytotoxic activity. As a result, the bioassay-guided fractionation led to the isolation of the mixture of the known compounds bafilomycin B-1 and bafilomycin B-2. The chemical structures of bafilomycin B-1 and bafilomycin B-2 were established based on their spectroscopic data by infrared (IR), mass spectrometry (MS), H-1 nuclear magnetic resonance (NMR), gradient-enhanced heteronuclear multiple quantum coherence (gHMQC), and gradient-enhanced heteronuclear multiple-bond connectivity (gHMBC) as well as comparison with reference data from the literature. Moreover, it was also possible to identify other bafilomycins using non-chromatographic-dependent techniques (Tandem mass spectrometry). Additionally, this is the first report on the phytotoxic activity of bafilomycin B-1. (AU)

FAPESP's process: 11/14333-6 - Diversity and polyphasic taxonomy of actinobacteria isolated from reforestation areas
Grantee:Tiago Domingues Zucchi
Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers