Ambient pH Sensing in Filamentous Fungi: Pitfalls in Elucidating Regulatory Hierarchical Signaling Networks

Autor(es)

Rossi, Antonio ^[1] ; Cruz, Aline H. S. ^[2] ; Santos, Rodrigo S. ^[2] ; Silva, Patricia M. ^[1] ; Silva, Emiliana M. ^[1] ; Mendes, Niege S. ^[2] ; Martinez-Rossi, Nilce M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IUBMB Life; v. 65, n. 11, p. 930-935, NOV 2013.
• Citações Web of Science: 10

Resumo

In this article, the experiments used to construct the ambient pH-signaling network involved in the secretion of enzymes by filamentous fungi have been reviewed, focusing on the phosphate-repressible phosphatases in Aspergillus nidulans. Classic and molecular genetics have been used to demonstrate that proteolysis of the transcription factor PacC at alkaline ambient pH is imperative for its action, implying that the full-length version is not an active molecular form of PacC. It has been hypothesized that the transcriptional regulator PacC may be functional at both acidic and alkaline ambient pH, in either the full-length or the proteolyzed form, if it carries a pal-dependent molecular tag. The products of the pal genes are involved in a metabolic pathway that led to the synthesis of effector molecules that tag the pacC product, perhaps facilitating its proteolysis. (c) 2013 IUBMB Life, 65(11):930-935, 2013 (AU)

• Processo FAPESP: 08/58634-7 - Genômica funcional e comparativa em fungos
• Beneficiário: Nilce Maria Martinez-Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/03689-7 - Mecanismos moleculares envolvidos no sensoriamento de fosfato inorgânico (Pi) em Neurospora crassa e em outros fungos.
• Beneficiário: Patrícia Martins da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado