Cytotoxic Effects of Thiamethoxam in the Midgut and Malpighian Tubules of Africanized Apis mellifera (Hymenoptera: Apidae)

Autor(es)

Catae, Aline Fernanda ^[1] ; Roat, Thaisa Cristina ^[1] ; De Oliveira, Regiane Alves ^[1] ; Ferreira Nocelli, Roberta Cornelio ^[2] ; Malaspina, Osmar ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Ctr Estudos Insetos Sociais, Inst Biociencias Rio Claro, Dept Biol, BR-13500900 Rio Claro, SP - Brazil

^[2] Ctr Ciencias Agr, Dept Ciencias Nat Matemat & Educ UFSCar, Araras, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 77, n. 4, p. 274-281, APR 2014.
• Citações Web of Science: 32

Resumo

Due to its expansion, agriculture has become increasingly dependent on the use of pesticides. However, the indiscriminate use of insecticides has had additional effects on the environment. These products have a broad spectrum of action, and therefore the insecticide affects not only the pests but also non-target insects such as bees, which are important pollinators of agricultural crops and natural environments. Among the most used pesticides, the neonicotinoids are particularly harmful. One of the neonicotinoids of specific concern is thiamethoxam, which is used on a wide variety of crops and is toxic to bees. Thus, this study aimed to analyze the effects of this insecticide in the midgut and Malpighian tubule cells of Africanized Apis mellifera. Newly emerged workers were exposed until 8 days to a diet containing a sublethal dose of thiamethoxam equal to 1/10 of LC50 (0.0428 ng a.i./l L of diet). The bees were dissected and the organs were processed for transmission electron microscopy. The results showed that thiamethoxam is cytotoxic to midgut and Malpighian tubules. In the midgut, the damage was more evident in bees exposed to the insecticide on the first day. On the eighth day, the cells were ultrastructurally intact suggesting a recovery of this organ. The Malpighian tubules showed pronounced alterations on the eighth day of exposure of bees to the insecticide. This study demonstrates that the continuous exposure to a sublethal dose of thiamethoxam can impair organs that are used during the metabolism of the insecticide. Microsc. Res. Tech. 77:274-281, 2014. (c) 2014 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 10/18460-0 - Análise histoquímica comparativa das glândulas de veneno de abelhas Apis mellifera africanizada e das vespas Polistes versicolor, Agelaia palipes palipes e Polybia paulista.
• Beneficiário: Aline Fernanda Catae
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/15628-7 - Análise morfológica e histoquímica do ventrículo e do cérebro de operárias recém-emergidas de Apis mellifera L. (Hymenoptera, Apidae) tratadas com Thiamethoxam
• Beneficiário: Regiane Alves de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/13370-8 - Como o cérebro de Apis mellifera (Lineu, 1758) (Hymenoptera, Apidae) responde à dose subletal de tiametoxam?
• Beneficiário: Thaisa Cristina Roat
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 08/05018-7 - Efeitos toxicológicos do inseticida fipronil em operárias e rainhas de Apis mellifera (Hymenoptera: Apidae): atividade neural e proteínas de desintoxicação
• Beneficiário: Thaisa Cristina Roat
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado