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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytotoxic Effects of Thiamethoxam in the Midgut and Malpighian Tubules of Africanized Apis mellifera (Hymenoptera: Apidae)

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Author(s):
Catae, Aline Fernanda [1] ; Roat, Thaisa Cristina [1] ; De Oliveira, Regiane Alves [1] ; Ferreira Nocelli, Roberta Cornelio [2] ; Malaspina, Osmar [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, UNESP, Ctr Estudos Insetos Sociais, Inst Biociencias Rio Claro, Dept Biol, BR-13500900 Rio Claro, SP - Brazil
[2] Ctr Ciencias Agr, Dept Ciencias Nat Matemat & Educ UFSCar, Araras, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 77, n. 4, p. 274-281, APR 2014.
Web of Science Citations: 32
Abstract

Due to its expansion, agriculture has become increasingly dependent on the use of pesticides. However, the indiscriminate use of insecticides has had additional effects on the environment. These products have a broad spectrum of action, and therefore the insecticide affects not only the pests but also non-target insects such as bees, which are important pollinators of agricultural crops and natural environments. Among the most used pesticides, the neonicotinoids are particularly harmful. One of the neonicotinoids of specific concern is thiamethoxam, which is used on a wide variety of crops and is toxic to bees. Thus, this study aimed to analyze the effects of this insecticide in the midgut and Malpighian tubule cells of Africanized Apis mellifera. Newly emerged workers were exposed until 8 days to a diet containing a sublethal dose of thiamethoxam equal to 1/10 of LC50 (0.0428 ng a.i./l L of diet). The bees were dissected and the organs were processed for transmission electron microscopy. The results showed that thiamethoxam is cytotoxic to midgut and Malpighian tubules. In the midgut, the damage was more evident in bees exposed to the insecticide on the first day. On the eighth day, the cells were ultrastructurally intact suggesting a recovery of this organ. The Malpighian tubules showed pronounced alterations on the eighth day of exposure of bees to the insecticide. This study demonstrates that the continuous exposure to a sublethal dose of thiamethoxam can impair organs that are used during the metabolism of the insecticide. Microsc. Res. Tech. 77:274-281, 2014. (c) 2014 Wiley Periodicals, Inc. (AU)

FAPESP's process: 10/18460-0 - Comparative histochemical analysis of the venom glands of africanized bees Apis mellifera and the wasps Polistes versicolor, Agelaia palipes palipes, Polybia paulista.
Grantee:Aline Fernanda Catae
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/15628-7 - Morphological and histochemical analysis of the midgut and brain from newly-emerged workers of Apis mellifera L. treated with Thiamethoxam
Grantee:Regiane Alves de Oliveira
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/13370-8 - How is the reaction of the Apis mellifera brain to a sublethal dose of thiamethoxam?
Grantee:Thaisa Cristina Roat
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 08/05018-7 - Toxicological effects of the insecticide fipronil in workers and queens of Apis mellifera (Hymenoptera: Apidae): neural activity and detoxification proteins
Grantee:Thaisa Cristina Roat
Support Opportunities: Scholarships in Brazil - Post-Doctoral