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Purification and characterization of antimicrobial peptides from fish isolate Carnobacterium maltaromaticum C2: Carnobacteriocin X and carnolysins A1 and A2

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Autor(es):
Tulini, Fabricio L. [1] ; Lohans, Christopher T. [2] ; Bordon, Karla C. F. [1] ; Zheng, Jing [2] ; Arantes, Eliane C. [1] ; Vederas, John C. [2] ; De Martinis, Elaine C. P. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
[2] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2 - Canada
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: International Journal of Food Microbiology; v. 173, p. 81-88, MAR 3 2014.
Citações Web of Science: 10
Resumo

Carnobacterium maltaromaticum C2, isolated from Brazilian smoked fish (Surubim, Pseudoplatystoma sp.), was found to exert antimicrobial activity against Listeria monocytogenes, an important foodborne pathogen. In this study, the bacteriocins produced by C. maltaromaticum 0 were purified via an extraction with XAD-16 resin, a C18 solid phase extraction, followed by reversed-phase fast protein liquid chromatography. The purified active fractions were characterized using tandem mass spectrometry, permitting the identification of multiple bacteriocins. Carnobacteriocins BM1, B1, and a variant of carnobacteriocin B2 were all found, providing much of the antilisterial activity. Additionally, we herein report the first isolation of the previously predicted antimicrobial peptide carnobacteriocin X. Moreover, C. maltaromaticum C2 produces a novel two-component lantibiotic, termed carnolysin, homologous to enterococcal cytolysin. This lantibiotic is antimicrobially inactive when tested against the non-bacteriocinogenic strain C. maltaromaticum A9b -, likely requiring an additional proteolytic cleavage to reach maturity. (C) 2013 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 09/10530-1 - Caracterização da bacteriocina de Carnobacterium maltaromaticum C2 isolado de peixe defumado brasileiro (surubim [Pseudoplatystoma sp.])
Beneficiário:Fabricio Luiz Tulini
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/11134-2 - Caracterização da bacteriocina de Carnobacterium maltaromaticum C2 isolado de peixe defumado brasileiro (Surubim [Pseudoplatystoma sp.])
Beneficiário:Elaine Cristina Pereira de Martinis
Modalidade de apoio: Auxílio à Pesquisa - Regular