Extraction of phenolic compounds from pitanga (Eugenia uniflora L.) leaves by sequential extraction in fixed bed extractor using supercritical CO2, ethanol and water as solvents

Autor(es)

Garmus, Tabata T. ^[1] ; Paviani, Losiane C. ^[1] ; Queiroga, Carmen L. ^[2] ; Magalhaes, Pedro M. ^[2] ; Cabral, Fernando A. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Dept Food Engn, BR-13083862 Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SUPERCRITICAL FLUIDS; v. 86, p. 4-14, FEB 2014.
• Citações Web of Science: 34

Resumo

With the goal of maximizing the extraction yield of phenolic compounds from pitanga leaves (Eugenia uniflora L), a sequential extraction in fixed bed was carried out in three steps at 60 degrees C and 400 bar, using supercritical CO2 (non-polar) as solvent in a first step, followed by ethanol (polarity: 5.2) and water (polarity: 9.0) in a second and third steps, respectively. All extracts were evaluated for global extraction yield, concentration and yield of both polyphenols and total flavonoids and antioxidant activity by DPPH method (in terms of EC50). The nature of the solvent significantly influenced the process, since the extraction yield increased with solvent polarity. The aqueous extracts presented higher global extraction yield (22%), followed by ethanolic (16%) and supercritical extracts (5%). The study pointed out that the sequential extraction process is the most effective in terms of global extraction yield and yield of polyphenols and total flavonoids, because it produced the more concentrated extracts on phenolic compounds, since the supercritical ethanolic extract presented the highest phenolics content (240.5 mg GAE/g extract) and antioxidant capacity (EC50 = 9.15 mu g/mL). The most volatile fraction from the supercritical extract, which is similar to the essential oils obtained by steam distillation or hydrodistillation, presented as major compounds the germacrenos D and B + bicyclogermacrene (40.75%), selina-13,7(11)-trien-8-one + selina-1,3,7(11)-trien-8-one epoxide (27.7%) and trans-caryophyllene (14.18%). (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/14309-8 - Obtenção de extratos de folhas de pitangueira (Eugenia uniflora L.) e de alecrim-pimenta (Lippia sidoides Cham.) por extração sequencial em leito fixo usando CO2 supercrítico, etanol e água como solventes
• Beneficiário: Tábata Tayara Garmus
• Modalidade de apoio: Bolsas no Brasil - Mestrado