Dendritic cells treated with chloroquine modulate experimental autoimmune encephalomyelitis

Autor(es)

Thome, Rodolfo ^[1] ; Issayama, Luidy Kazuo ^[1] ; DiGangi, Rosaria ^[1] ; Bombeiro, Andre Luis ^[1] ; da Costa, Thiago Alves ^[1] ; Ferreira, Isadora Tassinari ^[1] ; Rodrigues de Oliveira, Alexandre Leite ^[1] ; Verinaud, Liana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Immunology and Cell Biology; v. 92, n. 2, p. 124-132, FEB 2014.
• Citações Web of Science: 21

Resumo

Chloroquine (CQ), an antimalarial drug, has been shown to modulate the immune system and reduce the severity of experimental autoimmune encephalomyelitis (EAE). The mechanisms of disease suppression are dependent on regulatory T cell induction, although Tregs-independent mechanisms exist. We aimed to evaluate whether CQ is capable to modulate bone marrow-derived dendritic cells (DCs) both phenotypically and functionally as well as whether transfer of CQ-modulated DCs reduces EAE course. Our results show that CQ-treated DCs presented altered ultrastructure morphology and lower expression of molecules involved in antigen presentation. Consequently, T cell proliferation was diminished in coculture experiments. When transferred into EAE mice, DC-CQ was able to reduce the clinical manifestation of the disease through the modulation of the immune response against neuroantigens. The data presented herein indicate that chloroquine-mediated modulation of the immune system is achieved by a direct effect on DCs and that DC-CQ adoptive transfer may be a promising approach for avoiding drug toxicity. (AU)

• Processo FAPESP: 12/08303-0 - "Efeito da atrofia tímica induzida pela infecção com Plasmodium berghei no estabelecimento e curso da Encefalomielite Autoimune Experimental (EAE)"
• Beneficiário: Luidy Kazuo Issayama
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 11/13191-3 - Efeito da atrofia tímica induzida pela infecção com Plasmodium berghei sobre a indução e desenvolvimento da EAE experimental
• Beneficiário: Rodolfo Thomé
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/17965-3 - Efeito da atrofia tímica induzida pela infecção com Plasmodium berghei no estabelecimento e curso da encefalomielite autoimune experimental (EAE)
• Beneficiário: Liana Maria Cardoso Verinaud
• Modalidade de apoio: Auxílio à Pesquisa - Regular