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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dendritic cells treated with chloroquine modulate experimental autoimmune encephalomyelitis

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Author(s):
Thome, Rodolfo [1] ; Issayama, Luidy Kazuo [1] ; DiGangi, Rosaria [1] ; Bombeiro, Andre Luis [1] ; da Costa, Thiago Alves [1] ; Ferreira, Isadora Tassinari [1] ; Rodrigues de Oliveira, Alexandre Leite [1] ; Verinaud, Liana [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Immunology and Cell Biology; v. 92, n. 2, p. 124-132, FEB 2014.
Web of Science Citations: 21
Abstract

Chloroquine (CQ), an antimalarial drug, has been shown to modulate the immune system and reduce the severity of experimental autoimmune encephalomyelitis (EAE). The mechanisms of disease suppression are dependent on regulatory T cell induction, although Tregs-independent mechanisms exist. We aimed to evaluate whether CQ is capable to modulate bone marrow-derived dendritic cells (DCs) both phenotypically and functionally as well as whether transfer of CQ-modulated DCs reduces EAE course. Our results show that CQ-treated DCs presented altered ultrastructure morphology and lower expression of molecules involved in antigen presentation. Consequently, T cell proliferation was diminished in coculture experiments. When transferred into EAE mice, DC-CQ was able to reduce the clinical manifestation of the disease through the modulation of the immune response against neuroantigens. The data presented herein indicate that chloroquine-mediated modulation of the immune system is achieved by a direct effect on DCs and that DC-CQ adoptive transfer may be a promising approach for avoiding drug toxicity. (AU)

FAPESP's process: 11/13191-3 - Effect of thymic atrophy induced by infection with Plasmodium berghei on the induction and development of EAE experimental
Grantee:Rodolfo Thomé
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/17965-3 - Effect of thymic atrophy induced by Plasmodium berghei infection in the establishment and course of experimental autoimmune encephalomyelitis (EAE)
Grantee:Liana Maria Cardoso Verinaud
Support Opportunities: Regular Research Grants