Dynein c1h1, dynactin and syntaphilin expression in brain areas related to neurodegenerative diseases following exposure to rotenone

Autor(es)

Chaves, Rodrigo S. ^[1] ; Melo, Thaiany Q. ^[1] ; D'Unhao, Aline M. ^[1] ; Farizatto, Karen L. G. ^[1] ; Ferrari, Merari F. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA NEUROBIOLOGIAE EXPERIMENTALIS; v. 73, n. 4, p. 541-556, 2013.
• Citações Web of Science: 5

Resumo

Neurodegeneration is often accompanied by protein inclusions which may interfere with cell physiology. On the other hand, alteration in intracellular trafficking may precede impairment of neurotransmission and therefore trigger cell death. In view of this, it is hypothesized that changes in mitochondrial traffic may occur before neurodegeneration triggered by rotenone exposure and could favor this process. The effects of low concentrations of rotenone on the expression of dynein c1h1, dynactin and syntaphilin, which are proteins related to mitochondria transport and anchoring, were evaluated in cell cultures of substantia nigra, locus coeruleus and hippocampus as well as in these same brain areas in Lewis aged rats. The results indicate that low concentrations of rotenone decrease dynein c1h1 protein levels in cell cultures and brain areas of aged rats. Dynactin is decreased after exposure to 0.1 and 0.3 nM of rotenone, and increased after exposure to 0.5 nM of rotenone in cell cultures. Aged rats present increased dynactin expression. Syntaphilin expression decreased in vitro and increased in vivo after rotenone exposure. These findings suggest that changes in protein expression related to mitochondria. 1 retrograde transport and anchoring occur before neurodegeneration induced by rotenone exposure, which may be a primary factor to trigger neurodegenerative mechanisms. (AU)

• Processo FAPESP: 08/04480-9 - Análise do tráfego intracelular de mitocôndrias antes da formação de agregados protéicos em neurodegeneração
• Beneficiário: Merari de Fátima Ramires Ferrari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/12200-9 - Análise da expressão das proteínas RAB anterior à agregação proteica associada à neurodegeneração
• Beneficiário: Thaiany Quevedo Melo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/00478-2 - Influência do cálcio e das proteínas MIRO na mobilidade mitocondrial anteriormente e durante a agregação de proteínas envolvidas em neurodegeneração
• Beneficiário: Rodrigo dos Santos Chaves
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 11/06434-7 - Degradação da proteína tau hiperfosforilada e tráfego de organelas durante processos neurodegenerativos associados à agregação protéica em cultura de células do hipocampo
• Beneficiário: Merari de Fátima Ramires Ferrari
• Modalidade de apoio: Auxílio à Pesquisa - Regular