| Texto completo | |
| Autor(es): |
Moura Franco, Rita M. A. M.
[1]
;
Linhares, Marcelo M.
[1]
;
Lustosa, Suzana S.
[1]
;
Silva, Ismael D. G. C.
[1]
;
Souza, Naiara C. N.
[2]
;
Matos, Delcio
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, UNIFESP, Escola Paulista Med, EPM, BR-04288150 Sao Paulo - Brazil
[2] Piu XII Fdn, Barretos Canc Hosp, Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY; v. 7, n. 1, p. 255+, 2014. |
| Citações Web of Science: | 4 |
| Resumo | |
Background: Our objective was to examine how the gene expression profile of tumor tissue correlates with lymph node metastasis in patients with advanced colorectal adenocarcinoma (CRAC). Methods: We studied 36 patients (20 men and 16 women, 22-90 years of age) treated for CRAC (classifications of T2, T3, or T4; histological grade of G1 or G2). Amplified tumor mRNA samples were exposed to 20,000 human sequence probes and digitized images of the hybridized samples were analyzed. Results: On average, 2389 probes were detected above the background, with an average correlation R value of 0.19 between data from different patient groups (with or without lymph node invasion, colon or rectal, with or without angio-lymphatic invasion, with or without recurrence). Lymph node metastasis had a statistically significant signature according to Significance Analysis of Microarrays (SAM) and parametric t-tests, with a false discovery rate (FDR) = 0.1% and p = 0.001, respectively. Cross-correlation of these two tests identified 102 transcripts as being potentially related to node metastases, with fold changes in the range of 2.182-12.960. Conclusion: We identified 102 differentially expressed genes related to the presence of lymph node metastases in patients with advanced colorectal cancer. (AU) | |
| Processo FAPESP: | 04/10701-7 - Analise individual do perfil de expressao genica em doentes com adenocarcinoma colorretal, pela tecnica de microarrays tridimensionais de oligonucleotideos. |
| Beneficiário: | Delcio Matos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |