Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model

Autor(es):Mostrar menos -

Calio, Michele Longoni ^[1] ; Marinho, Darci Sousa ^[2] ; Ko, Gui Mi ^[2] ; Ribeiro, Renata Rodrigues ^[1] ; Carbonel, Adriana Ferraz ^[3] ; Oyama, Lila Missae ^[4] ; Ormanji, Milene ^[2] ; Guirao, Tatiana Pinoti ^[2] ; Calio, Pedro Luiz ^[5] ; Reis, Luciana Aparecida ^[6] ; Simoes, Manuel de Jesus ^[3] ; Lisboa-Nascimento, Telma ^[1] ; Ferreira, Alice Teixeira ^[1] ; Antonio Bertoncini, Clelia Rejane ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biofis, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Ctr Desenvolvimento Modelos Expt Med & Biol, BR-04023062 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Morfol, BR-04023062 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Fisiol, BR-04023062 Sao Paulo - Brazil

^[5] Univ Santa Cecilia, Dept Odontol, Santos, SP - Brazil

^[6] Univ Fed Sao Paulo, Dept Nefrol, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Free Radical Biology and Medicine; v. 70, p. 141-154, MAY 2014.
• Citações Web of Science: 57

Resumo

Stroke is the most common cause of motor disabilities and is a major cause of mortality worldwide. Adult stem cells have been shown to be effective against neuronal degeneration through mechanisms that include both the recovery of neurotransmitter activity and a decrease in apoptosis and oxidative stress. We chose the lineage stroke-prone spontaneously hypertensive rat (SHRSP) as a model for stem cell therapy. SHRSP rats can develop such severe hypertension that they generally suffer a stroke at approximately 1 year of age. The aim of this study was to evaluate whether mesenchymal stem cells (MSCs) decrease apoptotic death and oxidative stress in existing SHRSP brain tissue. The results of qRT-PCR assays showed higher levels of the antiapoptotic BcI-2 gene in the MSC-treated animals, compared with untreated. Our study also showed that superoxide, apoptotic cells, and by-products of lipid peroxidation decreased in MSC-treated SHRSP to levels similar those found in the animal controls, Wistar Kyoto rats. In addition, we saw a repair of morphological damage at the hippocampal region after MSC transplantation. These data suggest that MSCs have neuroprotective and antioxidant potential in stroke-prone spontaneously hypertensive rats. (c) 2014 The Authors. Published by Elsevier Inc. (AU)

• Processo FAPESP: 10/00106-5 - Estudo da diferenciação de células tronco mesenquimais e tumorais em células musculares e neuronais enfatizando-se a sinalização de cálcio e a expressão de marcadores após o transplante num modelo de acidente vascular cerebral
• Beneficiário: Alice Teixeira Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/60630-1 - Análise da expressão gênica de marcadores neurais em culturas de células-tronco adultas e possível aplicação da terapia celular para AVC em modelos "stroke prone" SHR
• Beneficiário: Michele Longoni Calió
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto