Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model

Author(s):Show less -

Calio, Michele Longoni ^[1] ; Marinho, Darci Sousa ^[2] ; Ko, Gui Mi ^[2] ; Ribeiro, Renata Rodrigues ^[1] ; Carbonel, Adriana Ferraz ^[3] ; Oyama, Lila Missae ^[4] ; Ormanji, Milene ^[2] ; Guirao, Tatiana Pinoti ^[2] ; Calio, Pedro Luiz ^[5] ; Reis, Luciana Aparecida ^[6] ; Simoes, Manuel de Jesus ^[3] ; Lisboa-Nascimento, Telma ^[1] ; Ferreira, Alice Teixeira ^[1] ; Antonio Bertoncini, Clelia Rejane ^[2]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biofis, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Ctr Desenvolvimento Modelos Expt Med & Biol, BR-04023062 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Morfol, BR-04023062 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Fisiol, BR-04023062 Sao Paulo - Brazil

^[5] Univ Santa Cecilia, Dept Odontol, Santos, SP - Brazil

^[6] Univ Fed Sao Paulo, Dept Nefrol, BR-04023062 Sao Paulo - Brazil

• Document type: Journal article
• Source: Free Radical Biology and Medicine; v. 70, p. 141-154, MAY 2014.
• Web of Science Citations: 57

Abstract

Stroke is the most common cause of motor disabilities and is a major cause of mortality worldwide. Adult stem cells have been shown to be effective against neuronal degeneration through mechanisms that include both the recovery of neurotransmitter activity and a decrease in apoptosis and oxidative stress. We chose the lineage stroke-prone spontaneously hypertensive rat (SHRSP) as a model for stem cell therapy. SHRSP rats can develop such severe hypertension that they generally suffer a stroke at approximately 1 year of age. The aim of this study was to evaluate whether mesenchymal stem cells (MSCs) decrease apoptotic death and oxidative stress in existing SHRSP brain tissue. The results of qRT-PCR assays showed higher levels of the antiapoptotic BcI-2 gene in the MSC-treated animals, compared with untreated. Our study also showed that superoxide, apoptotic cells, and by-products of lipid peroxidation decreased in MSC-treated SHRSP to levels similar those found in the animal controls, Wistar Kyoto rats. In addition, we saw a repair of morphological damage at the hippocampal region after MSC transplantation. These data suggest that MSCs have neuroprotective and antioxidant potential in stroke-prone spontaneously hypertensive rats. (c) 2014 The Authors. Published by Elsevier Inc. (AU)

• FAPESP's process: 10/00106-5 - Study of differentiation of the mesenchymal and tumoural cells into muscular and neuronal cells emphasizing calciun signalling and markers cells expression after transplantation in a stroke animal model
• Grantee: Alice Teixeira Ferreira
• Support Opportunities: Regular Research Grants