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Study of differentiation of the mesenchymal and tumoural cells into muscular and neuronal cells emphasizing calciun signalling and markers cells expression after transplantation in a stroke animal model


Adult stem cells (ASC) are responsible for integrity maintaining, repair and renew hurted tissue of adult organs. These cells are presents in the majory of tissues however, these cells were identifyed too a variety of cancers with similar caracteristics of ASC. ASC are used in many therapies of different etiology, as neurodegeneratives diseases, cerebral ischemic, cardiomyopathy, nervous system diseases and cancer. Netherless, due to the increase of these pathologies the use of cellular therapy could be a new stratgy to treat a variety of diseases, however it is necessary a deep study of ASC since is unclear the mechanisms that governs ASC the differentiation of these. In this way, the objective of this work are: 1- to caracteryze the mesenchymal SC (MSC) and the melanome SC, 2- to verify the transdifferentiation potential of MSC into neuron, 3- to analyse the applicability of the MSC in an acute cerebral stroke spontaneous model, 4- to observe the repair of radiobiologycal damages in smooth muscle cells by MSC, 5- to improve therapys eradicating the subpopulation of the melanoma MSC responsible for metastasis, and 6- to identify the calciun signaling via purinegycs, colinergycs nicotinics and glutamatergycs receptors stimulation in transdifferentiated cells from MSC e and melanome SC. We need to use RT-PCR techniques, light and confocal microscopy, flow cytometry, Patch Clamp and fluorimetry using fura-2/AM. This work will auxiliary the study of the repair development in an animal model of areas of acute cerebral stroke in the affected areas of, in the detachment and characterization of MSC and tumoral cells of melanome lineage, and in the transdifferentiation potential of MSC and melanoma SCs into functional neurons. This projecte will contribute to understand the molecular, morphology and functional bases of the mesenchymal and tumoral ASC and its differentiation into muscular and neuronal cells. This knowledge will be helpful to the future therapeutic identifying some aspects of the survival and proliferation celular in order to guide promising strategies of the cellular therapy. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CLARO, SANDRA; MIYAMOTO OSHIRO, MARIA ETSUKO; MORTARA, RENATO ARRUDA; PAREDES-GAMERO, EDGAR JULIAN; SILVA PEREIRA, GUSTAVO JOSE; SMAILI, SORAYA SOUBHI; FERREIRA, ALICE TEIXEIRA. gamma-Rays-generated ROS induce apoptosis via mitochondrial and cell cycle alteration in smooth muscle cells. International Journal of Radiation Biology, v. 90, n. 10, p. 914-927, . (10/00106-5)
CALIO, MICHELE LONGONI; MARINHO, DARCI SOUSA; KO, GUI MI; RIBEIRO, RENATA RODRIGUES; CARBONEL, ADRIANA FERRAZ; OYAMA, LILA MISSAE; ORMANJI, MILENE; GUIRAO, TATIANA PINOTI; CALIO, PEDRO LUIZ; REIS, LUCIANA APARECIDA; et al. Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model. Free Radical Biology and Medicine, v. 70, p. 141-154, . (10/00106-5)

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