ACUTE EFFECTS OF ESTRADIOL ON LUNG INFLAMMATION DUE TO INTESTINAL ISCHEMIC INSULT IN MALE RATS

Autor(es)

Breithaupt-Faloppa, Ana Cristina ^[1] ; Fantozzi, Evelyn Thais ^[2] ; Romero, Daniel Cancelli ^[2] ; Rodrigues, Adriana da Silva ^[2] ; Rocha de Sousa, Paulo Thales ^[1] ; dos Santos Franco, Adriana Lino ^[3] ; Oliveira-Filho, Ricardo Martins ^[2] ; Vargaftig, Bernardo Boris ^[2] ; de Lima, Wothan Tavares ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Cardiovasc Surg & Physiopathol Circulat LIM 1, Heart Inst InCor, Sch Med, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Shock; v. 41, n. 3, p. 208-213, MAR 2014.
• Citações Web of Science: 12

Resumo

Intestinal ischemia and reperfusion (intestinal I/R) causes acute lung inflammation that is characterized by leukocyte migration, increased lung microvascular permeability, and, in severe forms, noncardiogenic pulmonary edema and acute respiratory distress syndrome. Female sex hormones interfere with immune response, and experimental and clinical evidence shows that females are more resistant than males to organ injury caused by gut trauma. To reduce the lung inflammation caused by intestinal I/R, we have acutely treated male rats with estradiol. Intestinal I/R was performed by the clamping (45 min) of the superior mesenteric artery (SMA), followed by 2 h of intestinal reperfusion (unclamping SMA). Groups of rats received 17 beta estradiol (E2, 280 mu g/kg, i.v., single dose) 30 min after the SMA occlusion (ischemia period) or 1 h after the unclamping of SMA (reperfusion period). Leukocytes influx into the lung and microvascular leakage were assessed by lung myeloperoxidase activity and Evans blue dye extravasation, respectively. The lung expression of adhesion molecules (intercellular adhesion molecule 1, platelet endothelial cell adhesion molecule 1, and vascular cell adhesion molecule {[}VCAM]) was evaluated by immunohistochemistry. Interleukin 1 beta (IL-1 beta), IL-10, and NOx- concentrations were quantified in supernatants of cultured lung tissue. We have found that intestinal I/R increased the lung myeloperoxidase activity and Evans blue dye extravasation, which were reduced by treatment of rats with E2. Intestinal I/R increased ICAM-1 expression only, and it was decreased by E2 treatment. However, E2 treatment reduced the basal expression of platelet endothelial cell adhesion molecule 1. E2 treatment during intestinal ischemia was effective to reduce the levels of IL-10 and IL-1 beta in explant supernatant, but only IL-10 levels were reduced by E2 at reperfusion phase. The treatment with E2 did not affect NOx- concentration. Taken together, our data suggest that estradiol modulates the lung inflammatory response induced by lung injury, likely by acute effects. Thus, acute estradiol treatment could be considered as a potential therapeutic agent in ischemic events. (AU)

• Processo FAPESP: 09/54823-2 - Avaliacao do papel dos hormonios sexuais femininos e do sistema linfatico no controle da inflamacao pulmonar e sistemica apos a isquemica e reperfusao intestinal em ratas
• Beneficiário: Wothan Tavares de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular