Nanostructured Lipid Systems as a Strategy to Improve the in Vitro Cytotoxicity of Ruthenium(II) Compounds

Autor(es)

de Freitas, Eduardo Sinesio ^[1] ; da Silva, Patricia Bento ^[2] ; Chorilli, Marlus ^[2] ; Batista, Alzir Azevedo ^[3] ; Lopes, Erica de Oliveira ^[1] ; da Silva, Monize Martins ^[3] ; Fujimura Leite, Clarice Queico ^[1] ; Pavan, Fernando Rogerio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Farmacos & Medicamentos, BR-14801902 Sao Paulo - Brazil

^[3] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 19, n. 5, p. 5999-6008, MAY 2014.
• Citações Web of Science: 11

Resumo

Tuberculosis is an ancient disease that is still present as a global public health problem. Our group has been investigating new molecules with anti-TB activity. In this context, inorganic chemistry has been a quite promising source of such molecules, with excellent results seen with ruthenium compounds. Nanostructured lipid systems may potentiate the action of drugs by reducing the required dosage and side effects and improving the antimicrobial effects. The aim of this study was to develop a nanostructured lipid system and then characterize and apply these encapsulated compounds (SCARs 1, 2 and 4) with the goal of improving their activity by decreasing the Minimum Inhibitory Concentration (MIC90) and reducing the cytotoxicity (IC50). The nanostructured system was composed of 10% phase oil (cholesterol), 10% surfactant (soy oleate, soy phosphatidylcholine and Eumulgin (R)) and 80% aqueous phase (phosphate buffer pH = 7.4). Good activity against Mycobacterium tuberculosis was maintained after the incorporation of the compounds into the nanostructured lipid system, while the cytotoxicity decreased dramatically, in some cases up to 20 times less toxic than the unencapsulated drug. (AU)

• Processo FAPESP: 13/09265-7 - Avaliação do potencial de sistemas lipídicos nanoestruturados para administração de compostos de Cu(II) aplicáveis na otimização da terapia da tuberculose
• Beneficiário: Patrícia Bento da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/14957-5 - Investigação do potencial contra tuberculose de uma nova classe de compostos furoxânicos e compostos nanoestruturados de rutênio(II) e cobre(II)
• Beneficiário: Fernando Rogério Pavan
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores