Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line

Autor(es)

Carminati, Patricia Oliveira ^[1] ; Mello, Stephano Spano ^[1] ; Fachin, Ana Lucia ^[1] ; Junta, Cristina Moraes ^[1] ; Sandrin-Garcia, Paula ^[1] ; Carlotti, Carlos Gilberto ^[2] ; Donadi, Eduardo Antonio ^[3] ; Silva Passos, Geraldo Aleixo ^{[1, 4]} ; Sakamoto-Hojo, Elza Tiemi ^{[5, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cirugia & Anat, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Odontol Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 33, n. 1, p. 159-168, 2010.
• Citações Web of Science: 6

Resumo

Gliomas are the most common tumors in the central nervous system, the average survival time of patients with glioblastoma multiforme being about 1 year from diagnosis, in spite of harsh therapy. Aiming to study the transcriptional profiles displayed by glioma cells undergoing cisplatin treatment, gene expression analysis was performed by the cDNA microarray method. Cell survival and apoptosis induction following treatment were also evaluated. Drug concentrations of 12.5 to 300 μM caused a pronounced reduction in cell survival rates five days after treatment, whereas concentrations higher than 25 μM were effective in reducing the survival rates to ~1%. However, the maximum apoptosis frequency was 20.4% for 25 μM cisplatin in cells analyzed at 72 h, indicating that apoptosis is not the only kind of cell death induced by cisplatin. An analysis of gene expression revealed 67 significantly (FDR < 0.05) modulated genes: 29 of which down- and 38 up-regulated. These genes belong to several classes (metabolism, protein localization, cell proliferation, apoptosis, adhesion, stress response, cell cycle and DNA repair) that may represent several affected cell processes under the influence of cisplatin treatment. The expression pattern of three genes (RHOA, LIMK2 and TIMP2) was confirmed by the real time PCR method. (AU)

• Processo FAPESP: 99/12135-9 - Projeto transcriptoma: análise da expressão gênica em larga escala usando DNA-arrays
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 04/15611-6 - Mecanismos envolvidos nas respostas celulares aos compostos antitumorais e raios-gama em diferentes tipos de celulas humanas.
• Beneficiário: Elza Tiemi Sakamoto Hojo
• Modalidade de apoio: Auxílio à Pesquisa - Regular