Phenotypic Differences in Leucocyte Populations among Healthy Preterm and Full-Term Newborns

Autor(es)

Quinello, C. ^{[1, 2]} ; Silveira-Lessa, A. L. ^{[2, 3]} ; Ceccon, M. E. J. R. ^[1] ; Cianciarullo, M. A. ^[1] ; Carneiro-Sampaio, M. ^{[1, 2]} ; Palmeira, P. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Pediat, BR-05403900 Sao Paulo - Brazil

^[2] Hosp Clin Sao Paulo, Inst Crianca, Lab Med Invest LIM 36, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05403900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Scandinavian Journal of Immunology; v. 80, n. 1, p. 57-70, JUL 2014.
• Citações Web of Science: 12

Resumo

The immune system of neonates has been considered functionally immature, and due to their high susceptibility to infections, the aim of this study was to analyse the phenotypic differences in leucocyte populations in healthy preterm and full-term newborns. We evaluated the absolute numbers and frequencies of dendritic cells (DCs) and DC subsets, monocytes and T and B lymphocytes and subsets in the cord blood of healthy moderate and very preterm (Group 1), late preterm (Group 2) and full-term (Group 3) newborns and in healthy adults, as controls, by flow cytometry. The analyses revealed statistically higher absolute cell numbers in neonates compared with adults due to the characteristic leucocytosis of neonates. We observed a lower frequency of CD80+ myeloid and plasmacytoid DCs in Group 1 and reduced expression of TLR-4 on myeloid DCs in all neonates compared with adults. TLR-2+ monocytes were reduced in Group 1 compared with Groups 2 and 3, and TLR-4+ monocytes were reduced in Groups 1 and 2 compared with Group 3. The frequencies and numbers of naive CD4+ T and CD19+ B cells were higher in the three groups of neonates compared with adults, while CD4+ effector and effector memory T cells and CD19+ memory B cells were elevated in adults compared with neonates, as expected. Our study provides reference values for leucocytes in cord blood from term and preterm newborns, which may facilitate the identification of immunological deficiencies in protection against extracellular pathogens. (AU)

• Processo FAPESP: 09/52515-9 - Avaliacao dos mecanismos basicos de ativacao via receptores semelhantes ao toll 4 (tlr-4) em monocitos de recem nascidos a termo e pre-termo.
• Beneficiário: Ana Lúcia Silveira Lessa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/54246-5 - Avaliação da resposta imune neonatal: mecanismos básicos de ativação via receptores semelhantes ao Toll 2 e 4 (TLR-2 e 4) em diferentes células apresentadoras de antígenos de recém-nascidos a termo e pré-termo saudáveis e com sepse precoce e tardia
• Beneficiário: Patricia Palmeira Daenekas Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54400-4 - Avaliacao da resposta imune apos estimulacao de monocitos via toll-like receptor 2 (tlr-2) em recem-nascidos a termo e pre-termo.
• Beneficiário: Camila Cristina Quinello Gomes de Faria
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/53864-7 - EMU: plataforma de citometria de fluxo para implementação da investigação no Instituto da Criança do HC-FMUSP e no Instituto do Tratamento do Câncer Infantil (ITACI)
• Beneficiário: Magda Maria Sales Carneiro-Sampaio
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários