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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phenotypic Differences in Leucocyte Populations among Healthy Preterm and Full-Term Newborns

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Author(s):
Quinello, C. [1, 2] ; Silveira-Lessa, A. L. [2, 3] ; Ceccon, M. E. J. R. [1] ; Cianciarullo, M. A. [1] ; Carneiro-Sampaio, M. [1, 2] ; Palmeira, P. [1, 2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Med, Dept Pediat, BR-05403900 Sao Paulo - Brazil
[2] Hosp Clin Sao Paulo, Inst Crianca, Lab Med Invest LIM 36, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05403900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Scandinavian Journal of Immunology; v. 80, n. 1, p. 57-70, JUL 2014.
Web of Science Citations: 12
Abstract

The immune system of neonates has been considered functionally immature, and due to their high susceptibility to infections, the aim of this study was to analyse the phenotypic differences in leucocyte populations in healthy preterm and full-term newborns. We evaluated the absolute numbers and frequencies of dendritic cells (DCs) and DC subsets, monocytes and T and B lymphocytes and subsets in the cord blood of healthy moderate and very preterm (Group 1), late preterm (Group 2) and full-term (Group 3) newborns and in healthy adults, as controls, by flow cytometry. The analyses revealed statistically higher absolute cell numbers in neonates compared with adults due to the characteristic leucocytosis of neonates. We observed a lower frequency of CD80+ myeloid and plasmacytoid DCs in Group 1 and reduced expression of TLR-4 on myeloid DCs in all neonates compared with adults. TLR-2+ monocytes were reduced in Group 1 compared with Groups 2 and 3, and TLR-4+ monocytes were reduced in Groups 1 and 2 compared with Group 3. The frequencies and numbers of naive CD4+ T and CD19+ B cells were higher in the three groups of neonates compared with adults, while CD4+ effector and effector memory T cells and CD19+ memory B cells were elevated in adults compared with neonates, as expected. Our study provides reference values for leucocytes in cord blood from term and preterm newborns, which may facilitate the identification of immunological deficiencies in protection against extracellular pathogens. (AU)

FAPESP's process: 09/54246-5 - Evaluation of the neonatal immune response: basic mechanisms of activation via Toll-like receptors 2 and 4 (TLR-2 and 4) in different antigen presenting cells from healthy term and preterm newborns and with early and late onset sepsis
Grantee:Patricia Palmeira Daenekas Jorge
Support type: Regular Research Grants
FAPESP's process: 09/53864-7 - Flow cytometry for the Children’s Hospital and the Institute for Treatment of Childhood Cancer at the University of São Paulo School of Medicine (FM USP)
Grantee:Magda Maria Sales Carneiro-Sampaio
Support type: Multi-user Equipment Program