MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro

Autor(es)

Sipert, Carla R. ^[1] ; Morandini, Ana C. ^[1] ; Dionisio, Thiago J. ^[1] ; Trachtenberg, Alexander J. ^[2] ; Kuo, Winston P. ^{[3, 4]} ; Santos, Carlos F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[2] Harvard Univ, Sch Med, Harvard Catalyst Lab Innovat Translat Technol, Boston, MA - USA

^[3] Harvard Univ, Sch Med, Lab Innovat Translat Technol, Harvard Clin & Translat Sci Ctr, Boston, MA - USA

^[4] Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL SCIENCE; v. 56, n. 2, p. 157-164, JUN 2014.
• Citações Web of Science: 11

Resumo

MicroRNAs (miRNAs) are small non-coding RNAs showing a tissue-specific expression pattern, and whose function is to suppress protein synthesis. In this study, we hypothesized that expression of miRNAs would differ among fibroblasts from dental pulp (DPF), gingiva (GF) and periodontal ligament (PLF) in vitro. Once established by an explant technique, DPF, GF and PLF were collected for RNA isolation and subjected to a miRNA microarray. Next, cells were stimulated with E. coli lipopolysaccharide (LPS) for 24 h and then collected for RNA isolation. Expression of miR-146a and miR-155 was investigated by qPCR. Microarray screening revealed several miRNAs that showed specifically high expression in at least one of the fibroblast subtypes. These molecules are potentially involved in the regulation of extracellular matrix turnover and production of inflammatory mediators. Microarray analysis showed that both miR-146a and miR-155 were among the miRNAs expressed exclusively in GF. qPCR demonstrated significant upregulation of miR-146a only in GF after LPS stimulation, whereas basal expression of miR-155 was higher in GF than in the other cell subtypes. LPS downregulated the expression of miR-155 only in GF. Our results suggest that the expression and regulation of miR-146a and miR-155 are more pronounced in GF than in DPF and PLF. (AU)

• Processo FAPESP: 09/53848-1 - EMU: Aquisição de dois equipamentos de grande porte (Milliplex Analyzer Xponent 3 e acessórios e 7900 HT Fast Real Time PCR System e acessórios) para a realização de pesquisas por pesquisadores de diversos departamentos da FOB-USP e de outras instituições de pesquisa
• Beneficiário: Carlos Ferreira dos Santos
• Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 07/00306-1 - Expressão de pró-colágeno do tipo I, MIP-1alfa e SDF-1alfa por fibroblastos de gengiva e polpa dental humanas estimulados por ácido lipoteicóico de Enterococcus faecalis e lipopolissacarídeo de Escherichia coli e Porphyromonas gingivalis
• Beneficiário: Carla Renata Sipert
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 05/60167-0 - Expressão de pró-colágeno tipo 1, MIP-1± alfa e SDF-1 alfa por fibroblastos da polpa humana estimulados por ácido lipoteicóico de Streptococcus mutans
• Beneficiário: Carlos Ferreira dos Santos
• Linha de fomento: Auxílio à Pesquisa - Regular