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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro

Full text
Author(s):
Sipert, Carla R. [1] ; Morandini, Ana C. [1] ; Dionisio, Thiago J. [1] ; Trachtenberg, Alexander J. [2] ; Kuo, Winston P. [3, 4] ; Santos, Carlos F. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil
[2] Harvard Univ, Sch Med, Harvard Catalyst Lab Innovat Translat Technol, Boston, MA - USA
[3] Harvard Univ, Sch Med, Lab Innovat Translat Technol, Harvard Clin & Translat Sci Ctr, Boston, MA - USA
[4] Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 - USA
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF ORAL SCIENCE; v. 56, n. 2, p. 157-164, JUN 2014.
Web of Science Citations: 11
Abstract

MicroRNAs (miRNAs) are small non-coding RNAs showing a tissue-specific expression pattern, and whose function is to suppress protein synthesis. In this study, we hypothesized that expression of miRNAs would differ among fibroblasts from dental pulp (DPF), gingiva (GF) and periodontal ligament (PLF) in vitro. Once established by an explant technique, DPF, GF and PLF were collected for RNA isolation and subjected to a miRNA microarray. Next, cells were stimulated with E. coli lipopolysaccharide (LPS) for 24 h and then collected for RNA isolation. Expression of miR-146a and miR-155 was investigated by qPCR. Microarray screening revealed several miRNAs that showed specifically high expression in at least one of the fibroblast subtypes. These molecules are potentially involved in the regulation of extracellular matrix turnover and production of inflammatory mediators. Microarray analysis showed that both miR-146a and miR-155 were among the miRNAs expressed exclusively in GF. qPCR demonstrated significant upregulation of miR-146a only in GF after LPS stimulation, whereas basal expression of miR-155 was higher in GF than in the other cell subtypes. LPS downregulated the expression of miR-155 only in GF. Our results suggest that the expression and regulation of miR-146a and miR-155 are more pronounced in GF than in DPF and PLF. (AU)

FAPESP's process: 09/53848-1 - Acquisition of two large-capacity devices (a Milliplex Analyzer Xponent 3 and accessories and a 7900 HT Fast Real Time PCR System) for research conductedat the University of São Paulo School of Dentistry and at other research institutes
Grantee:Carlos Ferreira dos Santos
Support type: Multi-user Equipment Program
FAPESP's process: 07/00306-1 - Expression of procollagen type I, MIP-1alpha and SDF-1alpha by human gingival fibroblasts and dental pulp stimulated by Enterococcus faecalis lipoteichoic acid and lipopolysaccharide from Escherichia coli and Porphyromonas gingivalis
Grantee:Carla Renata Sipert
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 05/60167-0 - Expression of procollagen type 1, MIP-1± alpha and SDF-1 alpha by human fibroblasts stimulated pulp lipoteichoic acid from Streptococcus mutans
Grantee:Carlos Ferreira dos Santos
Support type: Regular Research Grants