Inhibition of peripheral anion exchanger 3 decreases formalin-induced pain

Autor(es)

Barragan-Iglesias, Paulino ^[1] ; Rocha-Gonzalez, Hector I. ^[2] ; Baruch Pineda-Farias, Jorge ^[1] ; Murbartian, Janet ^[1] ; Godinez-Chaparro, Beatriz ^[3] ; Reinach, Peter S. ^[4] ; Cunha, Thiago M. ^[4] ; Cunha, Fernando Q. ^[4] ; Granados-Soto, Vinicio ^[1]

Afiliação do(s) autor(es)

^[1] CINVESTAV, Dept Farmacobiol, Neurobiol Pain Lab, Ctr Invest & Estudios Avanzados, Mexico City 14330, DF - Mexico

^[2] Inst Politecn Nacl, Escuela Super Med, Secc Estudios Posgrad & Invest, Mexico City, DF - Mexico

^[3] Univ Autonoma Metropolitana Xochimilco, Dept Sistemas Biol, Div Ciencias Biol & Salud, Mexico City, DF - Mexico

^[4] Univ Sao Paulo, Riberao Preto Med Sch, Dept Pharmacol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Pharmacology; v. 738, p. 91-100, SEP 5 2014.
• Citações Web of Science: 4

Resumo

We determined the role of chloride-bicarbonate anion exchanger 3 in formalin-induced acute and chronic rat nociception. Formalin (1%) produced acute (first phase) and tonic (second phase) nociceptive behaviors (flinching and licking/lifting) followed by long-lasting evoked secondary mechanical allodynia and hyperalgesia in both paws. Local peripheral pre-treatment with the chloride-bicarbonate anion exchanger inhibitors 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid and 4-acetamido-4'-isothiocyanato-2,2'-stilbenedisulfonic acid prevented formalin-induced nociception mainly during phase 2. These drugs also prevented in a dose-dependent fashion long-lasting evoked secondary mechanical allodynia and hyperalgesia in both paws. Furthermore, post-treatment (on day 1 or 6) with 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid reversed established hypersensitivity. Anion exchanger 3 was expressed in dorsal root ganglion neurons and it co-localized with neuronal nuclei protein (NeuN), substance P and purinergic P2X3 receptors. Furthermore, Western blot analysis revealed a band of about 85 kDa indicative of anion exchanger 3 protein expression in dorsal root ganglia of naive rats, which was enhanced at 1 and 6 days after 1% formalin injection. On the other hand, this rise failed to occur during 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid exposure. These results suggest that anion exchanger 3 is present in dorsal root ganglia and participates in the development and maintenance of short and long-lasting formalin-induced nociception. (C) 2014 Elsevier By. All rights reserved. (AU)

• Processo FAPESP: 11/19670-0 - Mecanismos envolvidos na fisiopatologia da artrite reumatóide, dor e sepse
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Temático