| Full text | |
| Author(s): |
Barragan-Iglesias, Paulino
[1]
;
Rocha-Gonzalez, Hector I.
[2]
;
Baruch Pineda-Farias, Jorge
[1]
;
Murbartian, Janet
[1]
;
Godinez-Chaparro, Beatriz
[3]
;
Reinach, Peter S.
[4]
;
Cunha, Thiago M.
[4]
;
Cunha, Fernando Q.
[4]
;
Granados-Soto, Vinicio
[1]
Total Authors: 9
|
| Affiliation: | [1] CINVESTAV, Dept Farmacobiol, Neurobiol Pain Lab, Ctr Invest & Estudios Avanzados, Mexico City 14330, DF - Mexico
[2] Inst Politecn Nacl, Escuela Super Med, Secc Estudios Posgrad & Invest, Mexico City, DF - Mexico
[3] Univ Autonoma Metropolitana Xochimilco, Dept Sistemas Biol, Div Ciencias Biol & Salud, Mexico City, DF - Mexico
[4] Univ Sao Paulo, Riberao Preto Med Sch, Dept Pharmacol, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | European Journal of Pharmacology; v. 738, p. 91-100, SEP 5 2014. |
| Web of Science Citations: | 4 |
| Abstract | |
We determined the role of chloride-bicarbonate anion exchanger 3 in formalin-induced acute and chronic rat nociception. Formalin (1%) produced acute (first phase) and tonic (second phase) nociceptive behaviors (flinching and licking/lifting) followed by long-lasting evoked secondary mechanical allodynia and hyperalgesia in both paws. Local peripheral pre-treatment with the chloride-bicarbonate anion exchanger inhibitors 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid and 4-acetamido-4'-isothiocyanato-2,2'-stilbenedisulfonic acid prevented formalin-induced nociception mainly during phase 2. These drugs also prevented in a dose-dependent fashion long-lasting evoked secondary mechanical allodynia and hyperalgesia in both paws. Furthermore, post-treatment (on day 1 or 6) with 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid reversed established hypersensitivity. Anion exchanger 3 was expressed in dorsal root ganglion neurons and it co-localized with neuronal nuclei protein (NeuN), substance P and purinergic P2X3 receptors. Furthermore, Western blot analysis revealed a band of about 85 kDa indicative of anion exchanger 3 protein expression in dorsal root ganglia of naive rats, which was enhanced at 1 and 6 days after 1% formalin injection. On the other hand, this rise failed to occur during 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid exposure. These results suggest that anion exchanger 3 is present in dorsal root ganglia and participates in the development and maintenance of short and long-lasting formalin-induced nociception. (C) 2014 Elsevier By. All rights reserved. (AU) | |
| FAPESP's process: | 11/19670-0 - Mechanisms involved in the pathophysiology of rheumatoid arthritis, pain and sepsis |
| Grantee: | Fernando de Queiroz Cunha |
| Support Opportunities: | Research Projects - Thematic Grants |