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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Diet carotenoid lutein modulates the expression of genes related to oxygen transporters and decreases DNA damage and oxidative stress in mice

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Autor(es):
Serpeloni, Juliana Mara [1] ; de Syllos Colus, Ilce Mara [2] ; de Oliveira, Fabiola Singaretti [3] ; Aissa, Alexandre Ferro [1] ; Mercadante, Adriana Zerlotti [4] ; Pires Bianchi, Maria Lourdes [1] ; Greggi Antunes, Lusania Maria [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Sao Paulo - Brazil
[2] Univ Estadual Londrina, Dept Biol Geral, BR-86051990 Londrina, Parana - Brazil
[3] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Cirurgia Traumatol Bucomaxilofacial & Period, BR-14040903 Sao Paulo - Brazil
[4] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencia Alimentos, BR-13083862 Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Food and Chemical Toxicology; v. 70, p. 205-213, AUG 2014.
Citações Web of Science: 9
Resumo

Lutein (LT) is a carotenoid obtained by diet and despite its antioxidant activity had been biochemically reported, few studies are available concerning its influence on the expression of antioxidant genes. The expression of 84 genes implicated in antioxidant defense was quantified using quantitative reverse transcription polymerase chain reaction array. DNA damage was measured by comet assay and glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were quantified as biochemical parameters of oxidative stress in mouse kidney and liver. cDDP treatment reduced concentration of GSH and increased TBARS, parameters that were ameliorated in treatment associated with LT. cDDP altered the expression of 32 genes, increasing the expression of GPx2, APC, Nqo1 and CCs. LT changed the expression of 37 genes with an induction of 13 mainly oxygen transporters. In treatments associating cDDP and LT, 30 genes had their expression changed with a increase of the same genes of the cDDP treatment alone. These results suggest that LT might act scavenging reactive species and also inducing the expression of genes related to a better antioxidant response, highlighting the improvement of oxygen transport. This improved redox state of the cell through LT treatment could be related to the antigenotoxic and antioxidant effects observed. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/05096-8 - Atividade antigenotóxica de compostos da dieta e sua influência na expressão de genes de resposta ao estresse oxidativo
Beneficiário:Lusânia Maria Greggi Antunes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 05/59552-6 - Avaliação integrada da estabilidade e propriedades funcionais de pigmentos naturais de alimentos
Beneficiário:Adriana Zerlotti Mercadante
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/06793-4 - Atividade antigenotóxica de compostos da dieta e sua influência na expressão de genes de resposta ao estresse oxidativo
Beneficiário:Juliana Mara Serpeloni
Linha de fomento: Bolsas no Brasil - Doutorado