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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diet carotenoid lutein modulates the expression of genes related to oxygen transporters and decreases DNA damage and oxidative stress in mice

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Serpeloni, Juliana Mara [1] ; de Syllos Colus, Ilce Mara [2] ; de Oliveira, Fabiola Singaretti [3] ; Aissa, Alexandre Ferro [1] ; Mercadante, Adriana Zerlotti [4] ; Pires Bianchi, Maria Lourdes [1] ; Greggi Antunes, Lusania Maria [1]
Total Authors: 7
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Sao Paulo - Brazil
[2] Univ Estadual Londrina, Dept Biol Geral, BR-86051990 Londrina, Parana - Brazil
[3] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Cirurgia Traumatol Bucomaxilofacial & Period, BR-14040903 Sao Paulo - Brazil
[4] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencia Alimentos, BR-13083862 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Food and Chemical Toxicology; v. 70, p. 205-213, AUG 2014.
Web of Science Citations: 9

Lutein (LT) is a carotenoid obtained by diet and despite its antioxidant activity had been biochemically reported, few studies are available concerning its influence on the expression of antioxidant genes. The expression of 84 genes implicated in antioxidant defense was quantified using quantitative reverse transcription polymerase chain reaction array. DNA damage was measured by comet assay and glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were quantified as biochemical parameters of oxidative stress in mouse kidney and liver. cDDP treatment reduced concentration of GSH and increased TBARS, parameters that were ameliorated in treatment associated with LT. cDDP altered the expression of 32 genes, increasing the expression of GPx2, APC, Nqo1 and CCs. LT changed the expression of 37 genes with an induction of 13 mainly oxygen transporters. In treatments associating cDDP and LT, 30 genes had their expression changed with a increase of the same genes of the cDDP treatment alone. These results suggest that LT might act scavenging reactive species and also inducing the expression of genes related to a better antioxidant response, highlighting the improvement of oxygen transport. This improved redox state of the cell through LT treatment could be related to the antigenotoxic and antioxidant effects observed. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 05/59552-6 - Integrated evaluation of the stability and functional properties of natural pigments of foods
Grantee:Adriana Zerlotti Mercadante
Support type: Research Projects - Thematic Grants
FAPESP's process: 10/05096-8 - Antigenotoxic activity of compounds in diet and its effects in the expression of genes in response to oxidative stress
Grantee:Lusânia Maria Greggi Antunes
Support type: Regular Research Grants
FAPESP's process: 08/06793-4 - Antigenotoxic activity of compounds in the diet and its effect in the expression of genes in response to oxidative stress
Grantee:Juliana Mara Serpeloni
Support type: Scholarships in Brazil - Doctorate