Quantification of harman alkaloids in sour passion fruit pulp and seeds by a novel dual SBSE-LC/Flu (stir bar sorptive extraction-liquid chromatography with fluorescence detector) method

Autor(es)

Cíntia A. M. Pereira ; Thyago R. Rodrigues ^[2] ; Janete H. Yariwake ^[2]

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 25, n. 8, p. 1472-1483, 2014-08-00.

Resumo

A method for the quantification of the alkaloids harmane and harmine in sour passion fruit (Passiflora edulis f. flavicarpa O. Degener, Passifloraceae) pulp and seeds by stir-bar sorptive extraction and high performance liquid chromatography with fluorescence detection (dual SBSE-LC/Flu) is described. The SBSE parameters were optimized using a fractional factorial design, and the dual SBSE-LC/Flu method was validated following the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The high sensitivity and minimal sample handling of the dual SBSE-LC/Flu method make it attractive for application in phytochemical analysis or in the food industry. (AU)

• Processo FAPESP: 10/05711-4 - Aplicações da SBSE (SBSE/LC, SBSE/GC) ao estudo químico-analítico dos frutos de Passiflora alata e de P. edulis (maracujá), com ênfase para substâncias naturais potencialmente tóxicas
• Beneficiário: Janete Harumi Yariwake
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/59457-6 - Estudo químico-analítico de plantas medicinais e alimentícias brasileiras por HPLC e SBSE/LC
• Beneficiário: Janete Harumi Yariwake
• Modalidade de apoio: Auxílio à Pesquisa - Regular