Interactions of ascorbate bioflavonoids and redox biofactors in cytochrome c - induced peroxidative stress and apoptosis.

• Processo: 97/07865-2
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Data de Início da vigência: 01 de maio de 1998
• Data de Término da vigência: 30 de setembro de 2002
• Área de conhecimento: Ciências Biológicas - Bioquímica
• Pesquisador responsável: Brian John Bandy
• Beneficiário: Brian John Bandy
• Instituição Sede: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Vinculado ao auxílio: 96/00154-0 - Estresse oxidativo associado a porfírias caracterizadas por sobrecarga de ácido 5-aminolevulínico, AP.TEM
• Assunto(s): Apoptose Mitocôndrias
• Palavra(s)-Chave do Pesquisador: Apoptosis | Ascorbate | Bioflavonoids | Cytochrom C | Mitochondria | Peroxidations

Resumo

We propose investigations to extend preliminary observations that the bioflaronoid epicatechin rescued protection by ascorbate against peroxide-induced bleaching of cytochrome e that was prevented by liposomes of mitochondrial-like composition. Objectives: determine the prevalence of this interaction among bioflavonoids, and this influences of this inter-action and other redox factors on cytochrome c-catalyzed peroxidation's of liposomes and mitochondria. In addition we propose experiments to investigate the influences of peroxides, ascorbate, bioflavonoids and other redox factors on apoptosis triggered by cytochrome e release from mitochondrial membranes. (AU)