Interactions of ascorbate bioflavonoids and redox biofactors in cytochrome c - induced peroxidative stress and apoptosis.

• Grant number: 97/07865-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 1998
• End date: September 30, 2002
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Brian John Bandy
• Grantee: Brian John Bandy
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 96/00154-0 - Oxidative stress associated with porphyrias characterized by overload of 5-aminolevulinic acid, AP.TEM

Abstract

We propose investigations to extend preliminary observations that the bioflaronoid epicatechin rescued protection by ascorbate against peroxide-induced bleaching of cytochrome e that was prevented by liposomes of mitochondrial-like composition. Objectives: determine the prevalence of this interaction among bioflavonoids, and this influences of this inter-action and other redox factors on cytochrome c-catalyzed peroxidation's of liposomes and mitochondria. In addition we propose experiments to investigate the influences of peroxides, ascorbate, bioflavonoids and other redox factors on apoptosis triggered by cytochrome e release from mitochondrial membranes. (AU)