Association of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1, -2 and -9 during periapical lesion development

Autor(es)

Kalatzis Sousa, Natalia Guimaraes ^[1] ; de Barros Cardoso, Cristina Ribeiro ^[2] ; da Silva, Joeio Satana ^[3] ; Kuga, Milton Carlos ^[1] ; Tanomaru-Filho, Mario ^[1] ; Faria, Gisele ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal Toxicol & Food Sci, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Biochem & Immunol, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 59, n. 9, p. 944-953, SEP 2014.
• Citações Web of Science: 8

Resumo

Objective: To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice. Methods: Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 and 42 days the mandibles were removed. The periapical lesions were measured by micro-computed tomography. The expression of EMMPRIN, MMPs-1, -2, and -9 genes were determined by real-time RT-PCR. The location and expression of EMMPRIN and MMPs were evaluated by immunohistochemistry. Results: At 14 days, the periapical lesion area was higher than at 7 days. At 21 and 42 days no statistically significant bone loss was observed in comparison to 14 days. The control group showed discrete and occasional EMMPRIM, MMP-1, -2 and -9 immunostaining in the periodontal ligament fibroblasts. At 7, 14, 21 and 42 days intense immunoexpression was observed for EMMPRIN, MMPs-1, -2 and -9 in the region adjacent to the apical foramen. The EMMPRIN immunoexpression was higher at 7, 14, 21 and 42 days compared with the control. There was a positive correlation between gene expression of EMMPRIN and MMPs in the active phase of periapical lesion development. Conclusion: There is a high expression of EMMPRIM mainly by the inflammatory infiltrate in the region adjacent to the apical foramen during periapical lesion development. Furthermore, the positive correlation with MMP-1, -2, and -9 during the first days after periapical lesion induction indicates that EMMPRIM may be involved in the active phase of periapical lesions development. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/19511-0 - Resposta do tecido subcutâneo de camundongos frente a pasta triantibiótica indicada como medicação intracanal em dentes com necrose pulpar e rizogênese incompleta
• Beneficiário: Gisele Faria
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58064-6 - O possivel papel das metaloproteinases na patogenese da lesao periapical experimental
• Beneficiário: Gisele Faria
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado