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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Association of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1, -2 and -9 during periapical lesion development

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Author(s):
Kalatzis Sousa, Natalia Guimaraes [1] ; de Barros Cardoso, Cristina Ribeiro [2] ; da Silva, Joeio Satana [3] ; Kuga, Milton Carlos [1] ; Tanomaru-Filho, Mario [1] ; Faria, Gisele [1]
Total Authors: 6
Affiliation:
[1] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, BR-14801903 Araraquara, SP - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal Toxicol & Food Sci, BR-14040904 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Dept Biochem & Immunol, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 59, n. 9, p. 944-953, SEP 2014.
Web of Science Citations: 8
Abstract

Objective: To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice. Methods: Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 and 42 days the mandibles were removed. The periapical lesions were measured by micro-computed tomography. The expression of EMMPRIN, MMPs-1, -2, and -9 genes were determined by real-time RT-PCR. The location and expression of EMMPRIN and MMPs were evaluated by immunohistochemistry. Results: At 14 days, the periapical lesion area was higher than at 7 days. At 21 and 42 days no statistically significant bone loss was observed in comparison to 14 days. The control group showed discrete and occasional EMMPRIM, MMP-1, -2 and -9 immunostaining in the periodontal ligament fibroblasts. At 7, 14, 21 and 42 days intense immunoexpression was observed for EMMPRIN, MMPs-1, -2 and -9 in the region adjacent to the apical foramen. The EMMPRIN immunoexpression was higher at 7, 14, 21 and 42 days compared with the control. There was a positive correlation between gene expression of EMMPRIN and MMPs in the active phase of periapical lesion development. Conclusion: There is a high expression of EMMPRIM mainly by the inflammatory infiltrate in the region adjacent to the apical foramen during periapical lesion development. Furthermore, the positive correlation with MMP-1, -2, and -9 during the first days after periapical lesion induction indicates that EMMPRIM may be involved in the active phase of periapical lesions development. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/19511-0 - Subcutaneous tissue response of mice to triantibiotic paste indicated as intracanal medication for teeth with pulp necrosis and incomplete root formation
Grantee:Gisele Faria
Support Opportunities: Regular Research Grants