Isolation, detection, and immunomorphological characterization of circulating tumor cells (CTCs) from patients with different types of sarcoma using isolation by size of tumor cells: a window on sarcoma-cell invasion

Autor(es)

Domingos Chinen, Ludmilla T. ^[1] ; Lopes Mello, Celso A. ^[2] ; Abdallah, Emne Ali ^[1] ; Ocea, Luciana M. M. ^[1] ; Buim, Marcilei E. ^[1] ; Breve, Natalia M. ^[1] ; Gasparini Junior, Jose Luiz ^[1] ; Fanelli, Marcello F. ^[2] ; Paterlini-Brechot, Patrizia ^[3]

Afiliação do(s) autor(es)

^[1] AC Camargo Canc Ctr, Int Res Ctr, BR-01508010 Sao Paulo - Brazil

^[2] AC Camargo Canc Ctr, Dept Clin Oncol, BR-01508010 Sao Paulo - Brazil

^[3] Univ Paris 05, Unite INSERM U807, Paris - France

• Tipo de documento: Artigo Científico
• Fonte: ONCOTARGETS AND THERAPY; v. 7, p. 1609-1617, 2014.
• Citações Web of Science: 24

Resumo

Background: Sarcomas are rare and heterogeneous neoplasms with poor prognosis that are thought to spread to distant organs mainly by hematogenous dissemination. However, circulating tumor cells (CTCs) have never been visualized in sarcomas. Objectives: To investigate the feasibility of using isolation by size of tumor cells (ISET) for isolation, identification, and characterization of CTCs derived from patients with high-grade and metastatic sarcomas. Patients and methods: We studied eleven patients with metastatic/recurrent or locally advanced soft-tissue sarcomas (STSs), six of whom had synovial sarcomas. Blood samples (8 mL) were collected from patients with advanced STS and treated by ISET, a marker-independent approach that isolates intact CTCs from blood, based on their larger size compared with leukocytes. CTCs were identified by cytomorphology and characterized by dual-color immunocytochemistry using antivimentin or anti-Pan CK, and anti-CD45. Results: All patients with STS included in this study showed CTCs, with numbers ranging from two to 48 per 8 mL of blood. Conclusion: This study shows the feasibility of isolating, identifying, and characterizing CTCs from patients with different types of sarcomas and the presence of circulating sarcoma cells in all the tested patients. Our results set the basis for further studies aimed at exploring the presence, number, and immunomolecular characteristics of CTCs in different types of sarcoma, and bring more light to the mechanisms of tumor invasion for these tumors. (AU)

• Processo FAPESP: 12/01273-8 - Detecção de células tumorais circulantes e sua correlação com evolução clínica tumoral
• Beneficiário: Fernando Augusto Soares
• Linha de fomento: Auxílio à Pesquisa - Regular