One-week intervention period led to improvements in glycemic control and reduction in DNA damage levels in patients with type 2 diabetes mellitus

Autor(es)

Xavier, Danilo J. ^[1] ; Takahashi, Paula ^[1] ; Manoel-Caetano, Fernanda S. ^{[1, 2]} ; Foss-Freitas, Maria C. ^[3] ; Foss, Milton C. ^[3] ; Donadi, Eduardo A. ^{[1, 4]} ; Passos, Geraldo A. ^{[1, 5]} ; Sakamoto-Hojo, Elza T. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto FFCLR, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Med, Div Clin Immunol, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Fac Dent Ribeirao Preto, Dept Morphol, Disciplines Genet & Mol Biol, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Diabetes Research and Clinical Practice; v. 105, n. 3, p. 356-363, SEP 2014.
• Citações Web of Science: 6

Resumo

Aims: Hyperglycemia leads to increased production of reactive oxygen species (ROS), which reduces cellular antioxidant defenses and induces several DNA lesions. We investigated the effects on DNA damage of a seven-day hospitalization period in patients with type 2 diabetes mellitus (T2DM) to achieve adequate blood glucose levels through dietary intervention and medication treatment, compared with non-diabetic individuals. Methods: DNA damage levels were evaluated by the alkaline comet assay (with modified and without conventional use of hOGG1 enzyme, which detects oxidized DNA bases) for 10 patients and 16 controls. Real time PCR array method was performed to analyze the transcriptional expression of a set of 84 genes implicated in antioxidant defense and response to oxidative stress in blood samples from T2DM patients (n = 6) collected before and after the hospitalization period. Results: The seven-day period was sufficient to improve glycemic control and to significantly decrease (p < 0.05) DNA damage levels in T2DM patients, although those levels were slightly higher than those in control subjects. We also found a tendency towards a decrease in the levels of oxidative DNA damage in T2DM patients after the hospitalization period. However, for all genes analyzed, a statistically significant difference in the transcriptional expression levels was not observed. Conclusions: The study demonstrated that although the transcriptional expression of the genes studied did not show significant alterations, one-week of glycemic control in hospital resulted in a significant reduction in DNA damage levels detected in T2DM patients, highlighting the importance of an adequate glycemic control. (C) 2014 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/12069-7 - Perfis de expressão gênica e possíveis interações entre microRNAs e mRNAs em Diabetes Mellitus tipo 1 com enfoque em resposta ao estresse oxidativo e reparo do DNA
• Beneficiário: Paula Takahashi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/00932-2 - Perfis de expressão gênica associados ao processamento de dano oxidativo em pacientes com Diabetes Mellitus
• Beneficiário: Fernanda da Silva Manoel Caetano
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/56594-8 - Controle do transcriptoma no diabetes mellitus
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático