Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components

Autor(es)

de Oliveira, Haroldo Cesar ^[1] ; da Silva, Julhiany de Fatima ^[1] ; Matsumoto, Marcelo Teruyuki ^[1] ; Marcos, Caroline Maria ^[1] ; da Silva, Roberta Peres ^[1] ; Moraes da Silva, Rosangela Aparecida ^[1] ; Veneziano Labate, Monica Teresa ^[2] ; Labate, Carlos Alberto ^[2] ; Fusco Almeida, Ana Marisa ^[1] ; Soares Mendes Giannini, Maria Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Lab Micol Clin, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Lab Multiusuarios Ctr Genom Func Aplicada Agr & A, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Microbiology; v. 14, DEC 13 2014.
• Citações Web of Science: 6

Resumo

Background: Paracoccidioides spp is a fungi genus and the agent of paracoccidioidomycosis. The strategies of infection used by these pathogens involve the expression of proteins related to adaptation to the host, particularly regarding the uptake of micronutrients. This study analyzed the adhesion of Paracoccidioides lutzii during conditions of copper (Cu) and iron (Fe) deprivation, while also evaluating the proteins expressed in conditions of Cu depletion in the presence of four extracellular matrix (ECM) components (laminin, fibronectin and types I and IV collagen). Results: We cultured the P. lutzii in a chemically defined media without Cu and Fe. The fungus was then placed in contact with different ECM components and adhesion was evaluated. A significant increase in binding to all ECM components was observed when the fungus was cultured without Cu; which might be related to some adhesins expression. A proteomic assay was developed and revealed 39 proteins expressed that are involved in processes such as virulence, protein synthesis, metabolism, energy, transcription, transport, stress response and the cell cycle when the fungus was interacting with the ECM components. The up-regulated expression of two important adhesins, enolase and 14-3-3, was observed at the fungal cell wall during the interaction with the ECM components, indicating the role of these proteins in the Paracoccidioides-host interaction. Conclusions: This study is important for determining prospective proteins that may be involved in the interaction of Paracoccidioides with a host. Understanding the adaptive response to different growth conditions, elucidating the processes of adhesion and cell invasion, and identifying the proteins that are differentially expressed during the fungus-host interaction may help elucidate mechanisms used for survival and growth of Paracoccidioides in various human tissues. (AU)

• Processo FAPESP: 08/56476-5 - Influencia do cobre no padrao de expressao de genes envolvidos na interacao de paracoccidioides brasiliensis com a matriz extracelular.
• Beneficiário: Haroldo Cesar de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/18038-9 - Caracterização e análise funcional do gene da proteína 14-3-3 envolvida no processo de adesão do complexo Paracoccidioides sp. e identificação de alvos potenciais na terapia da paracoccidioidomicose
• Beneficiário: Maria José Soares Mendes Giannini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/52046-9 - Análise de genes diferencialmente expressos durante o contato de P. brasiliensis a componentes da matriz extracelular, pneumócitos e queratinócitos
• Beneficiário: Maria José Soares Mendes Giannini
• Modalidade de apoio: Auxílio à Pesquisa - Regular