| Full text | |
| Author(s): |
de Oliveira, Haroldo Cesar
[1]
;
da Silva, Julhiany de Fatima
[1]
;
Matsumoto, Marcelo Teruyuki
[1]
;
Marcos, Caroline Maria
[1]
;
da Silva, Roberta Peres
[1]
;
Moraes da Silva, Rosangela Aparecida
[1]
;
Veneziano Labate, Monica Teresa
[2]
;
Labate, Carlos Alberto
[2]
;
Fusco Almeida, Ana Marisa
[1]
;
Soares Mendes Giannini, Maria Jose
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Estadual Paulista, UNESP, Lab Micol Clin, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Lab Multiusuarios Ctr Genom Func Aplicada Agr & A, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | BMC Microbiology; v. 14, DEC 13 2014. |
| Web of Science Citations: | 6 |
| Abstract | |
Background: Paracoccidioides spp is a fungi genus and the agent of paracoccidioidomycosis. The strategies of infection used by these pathogens involve the expression of proteins related to adaptation to the host, particularly regarding the uptake of micronutrients. This study analyzed the adhesion of Paracoccidioides lutzii during conditions of copper (Cu) and iron (Fe) deprivation, while also evaluating the proteins expressed in conditions of Cu depletion in the presence of four extracellular matrix (ECM) components (laminin, fibronectin and types I and IV collagen). Results: We cultured the P. lutzii in a chemically defined media without Cu and Fe. The fungus was then placed in contact with different ECM components and adhesion was evaluated. A significant increase in binding to all ECM components was observed when the fungus was cultured without Cu; which might be related to some adhesins expression. A proteomic assay was developed and revealed 39 proteins expressed that are involved in processes such as virulence, protein synthesis, metabolism, energy, transcription, transport, stress response and the cell cycle when the fungus was interacting with the ECM components. The up-regulated expression of two important adhesins, enolase and 14-3-3, was observed at the fungal cell wall during the interaction with the ECM components, indicating the role of these proteins in the Paracoccidioides-host interaction. Conclusions: This study is important for determining prospective proteins that may be involved in the interaction of Paracoccidioides with a host. Understanding the adaptive response to different growth conditions, elucidating the processes of adhesion and cell invasion, and identifying the proteins that are differentially expressed during the fungus-host interaction may help elucidate mechanisms used for survival and growth of Paracoccidioides in various human tissues. (AU) | |
| FAPESP's process: | 11/18038-9 - Characterization and functional analysis of the gene for 14-3-3 protein involved in the process of adhesion of Paracoccidioides sp. complex and identification of potential targets in the treatment of paracoccidioidomycosis |
| Grantee: | Maria José Soares Mendes Giannini |
| Support Opportunities: | Regular Research Grants |