LINE-1 hypermethylation in peripheral blood of cutaneous melanoma patients is associated with metastasis

Autor(es)

De Araujo, Erica S. S. ^[1] ; Kashiwabara, Andre Y. ^[2] ; Achatz, Maria I. W. ^{[1, 3]} ; Moredo, Luciana F. ^[4] ; De Sa, Bianca C. S. ^[4] ; Duprat, Joao P. ^[4] ; Rosenberg, Carla ^[5] ; Carraro, Dirce M. ^[1] ; Krepischi, Ana C. V. ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Int Res Ctr, BR-05508090 Sao Paulo - Brazil

^[2] Fed Technol Univ Parana, Curitiba, Parana - Brazil

^[3] Univ Sao Paulo, Dept Oncogenet, BR-05508090 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Skin Canc Dept, AC Camargo Canc Ctr, BR-05508090 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Inst Biosci, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Melanoma Research; v. 25, n. 2, p. 173-177, APR 2015.
• Citações Web of Science: 12

Resumo

Aberrant DNA methylation pattern is a well-known epigenetic marker of cancer cells. Recently, aberrant methylation was also reported in the peripheral blood of cancer patients and it could potentially serve as a biomarker for cancer risk. We investigated the methylation pattern of LINE-1 and other repetitive DNA elements in peripheral blood of cutaneous melanoma patients in order to search for an association with clinical characteristics. The patient cohort was composed by 69 unrelated melanoma patients, 28 of whom were hereditary cases (with or without CDKN2A mutations) and 41 were isolated (sporadic) melanoma cases. Methylation of LINE-1 was evaluated by pyrosequencing, whereas additional repetitive DNA sequences were assessed using Illumina 450K methylation microarray. Melanoma patients exhibited a higher, albeit heterogeneous, LINE-1 methylation level compared with controls. Hereditary melanoma patients carrying CDKN2A mutations showed a hypermethylated pattern of both LINE-1 and repetitive DNA elements compared with other patients. In particular, the methylation level at one specific CpG of LINE-1 was found to be correlated with the occurrence of metastasis. Our data suggest that LINE-1 hypermethylation in peripheral blood of melanoma patients is a potential epigenetic biomarker for metastasis occurrence. Melanoma Res 25: 173-177 Copyright (C) 2015 Wolters Kluwer Health, Inc. All rights reserved. (AU)

• Processo FAPESP: 13/07480-8 - Fatores genéticos e epigenéticos na etiologia do melanoma cutâneo
• Beneficiário: Ana Cristina Victorino Krepischi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/13963-9 - Perfil global de metilação do genoma na síndrome do melanoma familial
• Beneficiário: Érica Sara Souza de Araújo
• Modalidade de apoio: Bolsas no Brasil - Doutorado