Ventilation causes pulmonary vascular dilation and modulates the NOS and VEGF pathway on newborn rats with CDH

Autor(es)

Gallindo, Rodrigo Melo ^[1] ; Lanhellas Goncalves, Frances Lilian ^[1] ; Figueira, Rebeca Lopes ^[1] ; Violin Dias Pereira, Luis Antonio ^[2] ; Bertoncini Simoes, Ana Leda ^[1] ; Schmidt, Augusto Frederico ^[3] ; Sbragia, Lourenco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Lab Expt Fetal Surg, Div Pediat Surg, BR-14048900 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Histol & Embryol, Campinas, SP - Brazil

^[3] Cincinnati Childrens Hosp Med Ctr, Cincinnati, OH 45229 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PEDIATRIC SURGERY; v. 50, n. 5, p. 842-848, MAY 2015.
• Citações Web of Science: 5

Resumo

Background/Purpose: Congenital diaphragmatic hernia (CDH) is a defect that presents high mortality because of pulmonary hypoplasia and hypertension. Mechanical ventilation changes signaling pathways, such as nitric oxide and VEGF in the pulmonary arterioles. We investigated the production of NOS2 and NOS3 and expression of VEGF and its receptors after ventilation in rat fetuses with CDH. Methods: CDH was induced by Nitrofen. The fetuses were divided into 6 groups: 1) control (C); 2) control ventilated (CV); 3) exposed to nitrofen (N-); 4) exposed to nitrofen ventilated (N-V), 5) CDH and 6) CDH ventilated (CDHV). Fetuses were harvested and ventilated. We assessed body weight (BW), total lung weight (TLW), TLW/BW ratio, the median pulmonary arteriolar wall thickness (MWT). We analyzed the expression of NOS2, NOS3, VEGF and its receptors by immunohistochemistry and Western blotting. Results: BW, TLW, and TLW/BWratiowere greater on C than on N- and CDH (p < 0.05). The MWT was higher in CDH than in CDHV (p < 0.001). CDHV showed increased expression of NOS3 (p < 0.05) and VEGFR1 (p < 0.05), but decreased expression of NOS2 (p < 0.05) and VEGFR2 (p < 0.001) compared to CDH. Conclusion: Ventilation caused pulmonary vasodilation and changed the expression of NOS and VEGF receptors. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/12587-0 - Avaliação in vivo do efeito do corticóide e da ventilação na via do óxido nítrico sobre a expressão do VEGF e seus receptores pulmonares após a oclusão traqueal fetal na hérnia diafragmática congênita em ratos
• Beneficiário: Frances Lilian Lanhellas Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/50347-9 - Efeito do ácido retinoico e sua interação com a traqueo-oclusão fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática em ratos
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/52772-9 - Efeito do acido retinoico sobre a expressao de receptores de vegf pulmonares em modelos animais de hernia diafragmatica congenita induzida por nitrofen.
• Beneficiário: Augusto Frederico Santos Schmidt
• Modalidade de apoio: Bolsas no Brasil - Mestrado