Ventilation causes pulmonary vascular dilation and modulates the NOS and VEGF pathway on newborn rats with CDH

Author(s)

Gallindo, Rodrigo Melo ^[1] ; Lanhellas Goncalves, Frances Lilian ^[1] ; Figueira, Rebeca Lopes ^[1] ; Violin Dias Pereira, Luis Antonio ^[2] ; Bertoncini Simoes, Ana Leda ^[1] ; Schmidt, Augusto Frederico ^[3] ; Sbragia, Lourenco ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Lab Expt Fetal Surg, Div Pediat Surg, BR-14048900 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Histol & Embryol, Campinas, SP - Brazil

^[3] Cincinnati Childrens Hosp Med Ctr, Cincinnati, OH 45229 - USA

• Document type: Journal article
• Source: JOURNAL OF PEDIATRIC SURGERY; v. 50, n. 5, p. 842-848, MAY 2015.
• Web of Science Citations: 5

Abstract

Background/Purpose: Congenital diaphragmatic hernia (CDH) is a defect that presents high mortality because of pulmonary hypoplasia and hypertension. Mechanical ventilation changes signaling pathways, such as nitric oxide and VEGF in the pulmonary arterioles. We investigated the production of NOS2 and NOS3 and expression of VEGF and its receptors after ventilation in rat fetuses with CDH. Methods: CDH was induced by Nitrofen. The fetuses were divided into 6 groups: 1) control (C); 2) control ventilated (CV); 3) exposed to nitrofen (N-); 4) exposed to nitrofen ventilated (N-V), 5) CDH and 6) CDH ventilated (CDHV). Fetuses were harvested and ventilated. We assessed body weight (BW), total lung weight (TLW), TLW/BW ratio, the median pulmonary arteriolar wall thickness (MWT). We analyzed the expression of NOS2, NOS3, VEGF and its receptors by immunohistochemistry and Western blotting. Results: BW, TLW, and TLW/BWratiowere greater on C than on N- and CDH (p < 0.05). The MWT was higher in CDH than in CDHV (p < 0.001). CDHV showed increased expression of NOS3 (p < 0.05) and VEGFR1 (p < 0.05), but decreased expression of NOS2 (p < 0.05) and VEGFR2 (p < 0.001) compared to CDH. Conclusion: Ventilation caused pulmonary vasodilation and changed the expression of NOS and VEGF receptors. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 11/12587-0 - Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats
• Grantee: Frances Lilian Lanhellas Gonçalves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen
• Grantee: Lourenço Sbragia Neto
• Support Opportunities: Regular Research Grants