Milk fermented with the probiotic candidate Lactobacillus paracasei FT700 induces differentiation of monocytes toward macrophages in vitro

Autor(es)

Tulini, Fabricio Luiz ^[1] ; Hymery, Nolwenn ^[2] ; Choiset, Yvan ^[3] ; Chobert, Jean-Marc ^[3] ; Haertle, Thomas ^[3] ; Pereira De Martinis, Elaine Cristina ^[1] ; Le Blay, Gwenaelle ^{[2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Brest, UEB, Lab Univ Biodiversite & Ecol Microbienne EA3882, ScInBioS SFR148 , ESIAB, Plouzane - France

^[3] INRA, UR 1268, Biopolymers Interact Assemblies, F-44316 Nantes 3 - France

^[4] Univ Brest, UEB, Inst Univ Europeen Mer IUEM UMR UBO, CNRS, IFREMER 6197, Lab Microbiol Environm Extremes, Plouzane - France

• Tipo de documento: Artigo Científico
• Fonte: Journal of Functional Foods; v. 15, p. 533-540, MAY 2015.
• Citações Web of Science: 1

Resumo

Lactobacillus paracasei FT700 was tested for its survival in simulated gastrointestinal conditions (in vitro) and for its sensitivity to antibiotics. The strain was used to produce fermented milk and the cell-free supernatant was freeze-dried and applied in cell cultures (monocytes and macrophages) to evaluate its cytotoxicity and immunomodulatory effects. After 180 min at pH 2.0 or in simulated gastric juice, L. paracasei FT700 concentrations were reduced by 4 and 3 log cfu/mL, respectively. Moreover, the strain was susceptible to the majority of antibiotics tested. Freeze dried cell-free fermented milk supematant (FMS) was toxic to monocytes after 72 h of exposure at 10 mg/mL inducing their apoptosis. However, at concentrations below toxic level, FMS stimulated the differentiation of monocytes into macrophages, as observed by the increased expression of CD71 marker. This immune stimulation was not inflammatory since low production of TNF-alpha was observed. Overall, L. paracasei FT700 has potential to be used as probiotic in functional fermented milk. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/11379-8 - Caracterização da alergenicidade e atividade antimicrobiana de proteínas do lactosoro após a ação de bactérias láticas isoladas de leite e queijo
• Beneficiário: Fabricio Luiz Tulini
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/11983-0 - Isolamento de bactérias láticas de leite e queijo com potencial para bioconservação de alimentos e utilização no aumento da digestibilidade de proteínas do lactosoro
• Beneficiário: Fabricio Luiz Tulini
• Modalidade de apoio: Bolsas no Brasil - Doutorado