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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Milk fermented with the probiotic candidate Lactobacillus paracasei FT700 induces differentiation of monocytes toward macrophages in vitro

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Author(s):
Tulini, Fabricio Luiz [1] ; Hymery, Nolwenn [2] ; Choiset, Yvan [3] ; Chobert, Jean-Marc [3] ; Haertle, Thomas [3] ; Pereira De Martinis, Elaine Cristina [1] ; Le Blay, Gwenaelle [2, 4]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Brest, UEB, Lab Univ Biodiversite & Ecol Microbienne EA3882, ScInBioS SFR148 , ESIAB, Plouzane - France
[3] INRA, UR 1268, Biopolymers Interact Assemblies, F-44316 Nantes 3 - France
[4] Univ Brest, UEB, Inst Univ Europeen Mer IUEM UMR UBO, CNRS, IFREMER 6197, Lab Microbiol Environm Extremes, Plouzane - France
Total Affiliations: 4
Document type: Journal article
Source: Journal of Functional Foods; v. 15, p. 533-540, MAY 2015.
Web of Science Citations: 1
Abstract

Lactobacillus paracasei FT700 was tested for its survival in simulated gastrointestinal conditions (in vitro) and for its sensitivity to antibiotics. The strain was used to produce fermented milk and the cell-free supernatant was freeze-dried and applied in cell cultures (monocytes and macrophages) to evaluate its cytotoxicity and immunomodulatory effects. After 180 min at pH 2.0 or in simulated gastric juice, L. paracasei FT700 concentrations were reduced by 4 and 3 log cfu/mL, respectively. Moreover, the strain was susceptible to the majority of antibiotics tested. Freeze dried cell-free fermented milk supematant (FMS) was toxic to monocytes after 72 h of exposure at 10 mg/mL inducing their apoptosis. However, at concentrations below toxic level, FMS stimulated the differentiation of monocytes into macrophages, as observed by the increased expression of CD71 marker. This immune stimulation was not inflammatory since low production of TNF-alpha was observed. Overall, L. paracasei FT700 has potential to be used as probiotic in functional fermented milk. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/11379-8 - Characterization of the allergenicity and antimicrobial activity of whey proteins after digestion by lactic acid bacteria isolated from milk and cheese
Grantee:Fabricio Luiz Tulini
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 11/11983-0 - Isolation of lactic acid bacteria from milk and cheese with potential for food biopreservation and utilization for increasing whey digestibility
Grantee:Fabricio Luiz Tulini
Support Opportunities: Scholarships in Brazil - Doctorate